中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 邻苯二甲酸二丁酯高效降解菌H-2的分离鉴定及其降解特性 食品科学 2014,35 (15) 202-206
Position: Home > Articles > Isolation and Identification of a Di-n-butyl phthalate(DBP)-Degrading Strain H-2 and Its Degradation Characteristics FOOD SCIENCE 2014,35 (15) 202-206

邻苯二甲酸二丁酯高效降解菌H-2的分离鉴定及其降解特性

作  者：
金雷;陈瑜;严忠雍;郭远明;祝银;龙举;顾蓓乔
单  位：
浙江省海洋水产研究所
关键词：
邻苯二甲酸二丁酯;类芽孢杆菌属;降解特性;降解谱
摘  要：
为了获得高效广谱的邻苯二甲酸二丁酯(di-n-butyl phthalate,DBP)降解菌,本研究从长期受食品塑料垃圾污染的土壤中通过富集培养和分离纯化,获得一株DBP高效降解菌,命名为H-2。根据菌株的形态、生理生化特征和16S rRNA序列分析,将其鉴定为类芽孢杆菌属(Paenibacillus sp.)。运用高效液相色谱法分析了菌株H-2对DBP的降解特性及对邻苯二甲酸酯类化合物的广谱性降解情况。结果表明:菌株H-2降解DBP的最适温度为30℃,最适pH值为7.0;在此最适条件下,菌株H-2在3 d内对100 mg/L DBP的降解率高达87.6%。菌株H-2能高效降解短链邻苯二甲酸二甲酯、邻苯二甲酸二乙酯和DBP,而对长链邻苯二甲酸二辛酯的降解效果较差。
译  名：
Isolation and Identification of a Di-n-butyl phthalate(DBP)-Degrading Strain H-2 and Its Degradation Characteristics
作  者：
JIN Lei;CHEN Yu;YAN Zhong-yong;GUO Yuan-ming;ZHU Yin;LONG Ju;GU Bei-qiao;Marine Fishery Research Institute of Zhejiang Province;Zhejiang Province Key Laboratory of Mariculture and Enhancement;
关键词：
di-n-butyl phthalate;;Paenibacillus sp.;;degradation characteristics;;degradation spectrum
摘  要：
A broad-spectrum and efficient di-n-butyl phthalate(DBP)-degrading bacterial strain H-2 was isolated from plastic food packaging garbage-contaminated soil by enrichment culture and purification. Based on its morphological, physiobiochemical characteristicsand 16 S rRNA gene sequence, strain H-2 was identified as Paenibacillus sp. By high performance liquid chromatography(HPLC), its DBP degrading characteristics and diversity of de gradable substrates were studied. The results showed that the optimal temperature and pH for DBP degradation by strain H-2 was 30 ℃ and 7.0, respectively. Under these conditions, H-2 degraded more than 87.6% of 100 mg/L DBP within 3 d. The diversity of degradable substrates showed that strain H-2 could degrade phthalate(DMP), DEP and DBP efficiently, while DOP was degraded difficultly by strain H-2. This research may provide the theoretical basis to decontaminate PAEs pollution of plastic food packaging garbage.

相似文章

导出引用
计量
文章访问数: 8
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46