译 名：
Optimization of Dual-Enzymatic Hydrolysis for Protein Extraction from Sloanea hemsleyana Seeds
作 者：
MA Cheng-jin 1,2,HUANG Wei 2,HUANG Qun 2,3,FENG Lei 2,WU Li-ya 2,3,SU Chao 2(1.Key Laboratory of Hunan Forest Products and Chemical Industry Engineering,Jishou University,Zhangjiajie 427000,China;2.Institute of Food Science,Jishou University,Jishou 416000,China;3.Key Laboratory of Plant Resources Conservation and Utilization of Hunan Province,Jishou University,Jishou 416000,China)
关键词：
Sloanea hemsleyana seed protein;extraction;dual-enzymatic hydrolysis;quadratic regression universal rotary combination design
摘 要：
Dual-enzymatic hydrolysis of Sloanea hemsleyana seeds with cellulase and papain was the best way for protein extraction as demonstrated by a comparison of the hydrolysis efficiencies of 6 different enzymes on Sloanea hemsleyana seeds.The effects of simultaneous or stepwise hydrolysis,cellulase/papain ratio,solid/solvent ratio,total enzyme dosage,temperature,hydrolysis time and pH on protein yield were investigated using one-factor-at-a-time design.Further,total enzyme dosage,temperature,hydrolysis time and pH were optimized using quadratic regression universal rotary combination design.The optimal hydrolysis conditions for protein extraction from Sloanea hemsleyana seeds were determined as follows: Sloanea hemsleyana seed meal was suspended in deionized water at a solid/liquid ratio of 1:25,and added simultaneously with cellulase and papain at a ratio of 6:4 until a total enzyme dose of 370 U/g for hydrolysis at pH 7.6,57 ℃ for 80 min.Under these conditions,the extraction yield of protein from Sloanea hemsleyana seeds was 74.91%.
