单 位：
吉首大学植物资源保护与利用湖南省高校重点实验室;吉首大学食品科学研究所
关键词：
仿栗籽蛋白;碱提酸沉;响应面法;提取工艺
摘 要：
研究了仿栗籽蛋白的碱溶酸沉法提取工艺。在单因素实验的基础上,选定提取温度、pH、提取时间、液料比四个因素的三个水平进行中心组合实验,建立了仿栗籽蛋白提取率的二次回归方程,通过响应面分析以及岭嵴分析得到了优化组合条件。实验结果表明,当提取工艺条件为pH9.6、温度42℃、时间138min、料液比1∶25(g/mL)时,仿栗籽蛋白提取率为64.97%。
译 名：
Optimization of the extraction technology of protein from sloanea hemsleyana seed by response surface methodology
作 者：
HUANG Wei1,2,MA Cheng-jin1,2,*,HUANG Qun2,SU Chao2,FENG Lei2 (1. Key Laboratory of Plant Resources Conservation and Utilization,College of Hunan Province,Jishou University,Jishou 416000,China; 2. Institute of Food Science,Jishou University,Jishou 416000,China)
关键词：
sloanea hemsleyana seed protein; alkaline extraction and acid precipitation; response surface methodology;extraction process
摘 要：
The extraction of protein from the sloanea hemsleyana seed by alkaline extraction and acid precipitation was studied. According to the single-factor experiments,three levels of the factors(extraction temperature,extraction time,pH and material/liquid ratio) were selected for the central composite design to establish a quadric regression equation for describing the yield of sloanea hemsleyana seed protein. By response surface analysis,the optimum extraction conditions were obtained as follows:extraction temperature of 42℃,time of 138min,pH9.6 and material/liquid ratio of 1∶25(g/mL),under which the highest yield of sloanea hemsleyana seed protein reached 64.97%.
