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Position: Home > Articles > Study on Gas Chromatography Detection Method of Trans Fatty Acids in Food FOOD SCIENCE 2008,29 (12) 511-515

气相色谱检测食品中反式脂肪酸方法的研究

作  者：
宋立华;陆小花;杜韩香;林超
单  位：
上海交通大学农业与生物学院食品科学与工程系
关键词：
反式脂肪酸;毛细管气相色谱
摘  要：
实验研究了不同色谱条件下食品中常见的反油酸、反异油酸及反亚油酸的分离效果,确定的色谱条件为:HP-88毛细管色谱柱(60m×0.25mm,0.2μm);柱温:恒温180℃;检测器:250℃;进样器:250℃;载气:高纯氮,压力:100kPa,流速:16ml/min;氢气压力:100kPa,流速:28ml/min;空气压力:160kPa,流速:200ml/min;分流比:30:1;尾吹流速:18ml/min,在此操作条件下30min内能较好的分离顺/反式油酸甲酯(C18:1,c9/C18:1,t9)及其同分异构体反异油酸甲酯(C18:1,t11)和顺/反式亚油酸甲酯(C18:2,c9c12/C18:2,t9t12)。在0.05～0.4mg/ml浓度范围内线性良好(r>0.998);三种反式脂肪酸的检测限分别为:6.84×10-15、8.28×10-15、6.57×10-15g/s。从甲酯化处理至气相色谱检测,方法的回收率在85.29%～86.41%范围间,相对标准偏差在1.47%～8.50%之间。
译  名：
Study on Gas Chromatography Detection Method of Trans Fatty Acids in Food
作  者：
SONG Li-hua1,2,LU Xiao-hua1,DU Han-xiang1,LIN Chao1(1.Department of Food Science and Engineer,Agricultural and Biological College,Shanghai Jiao Tong University,Shanghai 201101,China;2.SJTU-Bor S.Luh Food Safety,Agricultural and Biological College,Shanghai Jiao Tong University,Shanghai 201101,China)
关键词：
trans-fatty acid;gas chromatography(GC)
摘  要：
In the present study,the separatation effects of different elaidic acid,trans-vaccenic acid,and linolelaidic acid mainly contained in food under different gas chromatography(GC) conditions. It was found experimentally that the optimal GC condition are as follows:HP-88 capillary column(60m×0.25mm,0.2 μ m),column temperature 180 ℃; sampler temperature:250 ℃,detector temperature:250 ℃,pure N2 as carrier gas,pure N2 pressure 100kPa,plue N2 flow rate:16 ml/min,hydrogen pressure 100 kPa,hydrogen flow rate 28ml/min,air pressure 160kPa,air flow rate:200 ml/min,split injection mode with the split ratio of 30:1 and tail-blows rate 18 ml/min. Elaidic acid(C18:1,t9)、linolelaidic acid(C18:2,t9 t12)and trans-vaccenic acid(C18:1,t11) can be separated completely within 30 min under these operation conditions. And the linear scopes are between 0.05mg/ml and 0.4mg/ml(r>0.998). The detection limits of the three trans fatty acid are 6.84×10-15 g/s,8.28×10-15 g/s,and 6.57×10-15 g/s,respectively. The recovery rate of the method from esterification to GC detection is between 85.29% and 86.41% with the relative standard deviation is between 1.47%and 8.50%.

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