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当前位置: 首页 > 文章 > 拜城油鸡公鸡A-FABP基因多态性及其与生产性能的相关性分析 中国畜牧兽医 2017,44 (1) 141-147
Position: Home > Articles > Polymorphism of A-FABP Gene and Its Association Analysis with the Productive Performance in Male Baicheng Fatty Chicken China Animal Husbandry & Veterinary Medicine 2017,44 (1) 141-147

拜城油鸡公鸡A-FABP基因多态性及其与生产性能的相关性分析

作  者：
邵勇钢;臧长江;曾伟丹;王彦钦
单  位：
新疆农业大学动物科学学院
关键词：
拜城油鸡;A-FABP基因;多态性;生产性能
摘  要：
试验旨在研究拜城油鸡公鸡中脂肪型脂肪酸结合蛋白(adipocyte fatty acid binding protein,A-FABP)基因的多态性及其与生产性能的相关性,寻找可用于拜城油鸡选育的分子遗传标记。随机选取92只6周龄拜城油鸡公鸡,采用PCR-RFLP方法检测A-FABP基因的多态性。每种基因型选取15只体重相近的个体随机分为3组,每组每种基因型个体各5只,分别在12、18、24周龄时屠宰并对生产性能指标进行测定。结果显示,在拜城油鸡公鸡中检测到A-FABP基因有3种基因型:AA、AB、BB,BB为优势基因型,B为优势等位基因,不符合哈代-温伯格平衡定律(P<0.01)。12周龄时,各基因型间各生产性能指标差异均不显著(P>0.05);18周龄时,BB基因型肌间脂宽显著高于AA基因型(P<0.05),BB和AB基因型腹脂重极显著高于AA基因型(P<0.01),腹脂率显著高于AA基因型(P<0.05);24周龄时,BB基因型体重、肌间脂宽、腹脂重均显著高于AA基因型(P<0.05)。推测A-FABP基因可以作为与拜城油鸡公鸡脂肪性状相关的候选标记之一。
译  名：
Polymorphism of A-FABP Gene and Its Association Analysis with the Productive Performance in Male Baicheng Fatty Chicken
作  者：
SHAO Yong-gang;ZANG Chang-jiang;ZENG Wei-dan;WANG Yan-qin;College of Animal Science,Xinjiang Agricultural University;
关键词：
Baicheng fatty chicken;;A-FABP gene;;polymorphism;;productive performance
摘  要：
In order to search the molecular genetic markers for breeding of Baicheng fatty chicken,the relationship between the polymorphism of adipocyte fatty acid binding protein(A-FABP)gene and the productive performance of the male chickens at different ages was studied.Ninety two chickens with age of 6weeks were randomly selected.The polymorphism of A-FABP gene was detected by PCR-RFLP method.In each genotype group,fifteen chickens with similar body weight were categorized,each group was randomly divided into three sub-groups with equal numbers.The productive performance of the chickens was measured at their age of 12,18 and 24weeks.The results showed that there were three genotypes of A-FABP gene:AA,AB and BB,BB genotype was the dominant genotype,allele B was the advantageous allele,and it was not in Hardy-Weinberg equilibrium(P<0.01).At the age of 12 weeks,no significant difference was found among the measured productive performance(P>0.05).At the age of 18 weeks,it was found that the intramuscular fat width of BB genotype was significantly higher than AA genotype(P<0.05),the abdominal fat weight of BB and AB genotypes were extremely significantly higher than AA genotype(P<0.01),and the abdominal fat weight percentage of BB and AB genotypes were significantly higher than AA genotype(P<0.05).At the age of 24 weeks,it wasfound that the body weight,intramuscular fat width and abdominal fat weight of BB genotype were significantly higher than that of AA genotype(P<0.05).Therefore,this results suggested that A-FABPgene could be used as a potential candidate marker for the association of fatty traits in male Baicheng fatty chicken.

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