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当前位置: 首页 > 文章 > 猪带绦虫45W基因家族cDNA克隆和序列分析 中国农业科学 2004,37 (5) 776-780
Position: Home > Articles > Cloning and Sequencing of Complementary DNA of 45 WGene Family from Taenia solium Scientia Agricultura Sinica 2004,37 (5) 776-780

猪带绦虫45W基因家族cDNA克隆和序列分析

作  者：
贾万忠;郑亚东;王佩雅;陈涓;程晓红;景志忠;才学鹏
单  位：
中国农业科学院兰州兽医研究所
关键词：
猪带绦虫;45W基因;cDNA
摘  要：
根据猪带绦虫45W基因序列设计引物,用反转录-聚合酶链式反应(RT-PCR)对六钩蚴总RNA进行cDNA扩增,扩增产物经琼脂糖凝胶电泳分离、纯化、回收,与载体pGEMT-easyVector连接,转化大肠杆菌JM109,筛选阳性克隆,测序。结果显示,已成功克隆到重要保护性抗原45W基因B型转录本cDNA,完整开放阅读框(ORF)的大小为459bp。序列同源性分析与比较表明,所获得的9个B型转录本cDNA除TSO45W-4B-1和TSO45W-4B-2与已报道的TSO45W-4B同源外,其余均属于首次报道的45W基因家族新成员。各cDNA克隆之间核苷酸序列的差异性为1.5%～19.8%,氨基酸序列之间的差异性为2.6%～29.7%,后者比前者的差异程度更大。
译  名：
Cloning and Sequencing of Complementary DNA of 45 WGene Family from Taenia solium
作  者：
JIA Wan-zhong,ZHENG Ya-dong,WANG Pei-ya,CHEN Juan,CHENG Xiao-hong,JING Zhi-zhong,CAI Xue-peng(Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046)
关键词：
Taenia solium;45W gene;cDNA sequence
摘  要：
Complementary DNAs (cDNAs) of 45W gene family were amplified from total RNA of hatched and activatedTaenia solium oncosphere using reverse transcription polymerase chain reaction (RT-PCR). The agarose gel electrophoresisof the PCR products showed that the target DNA fragments were specifically amplified. The fragments (492bp) werepurified using DNA purification kit, inserted into pGEM-T Easy Vector, and transformed into JM 109 and then sequenced.The relative sequences were retrieved from GenBank and aligned with our data by DNAstar software. The results showedthat the cDNAs of 9 B-type transcripts of T. solium have been obtained with a 459 bp open reading frame. These transcriptsbelonged to the T. solium 45W gene family with 1.5%-19.8% nucleotide divergence and 2.5%-29.7% amino acid residuedivergence. 7 of 9 transcripts were the new members reported at the first time, while the others almost had the samesequences as TSO45W-4B reported in GenBank.

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