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当前位置: 首页 > 文章 > 长春花高效快繁技术研究 安徽农业科学 2009,37 (22) 10404-10406
Position: Home > Articles > Rapid Propagation Techniques of Catharanthus roseus(L.) G.Don Journal of Anhui Agricultural Sciences 2009,37 (22) 10404-10406

长春花高效快繁技术研究

作  者：
陈泉;李建国
单  位：
海南大学农学院;中国热带农业科学院分析测试中心
关键词：
长春花;种子消毒;增殖和生长;生根诱导
摘  要：
[目的]建立长春花高效的组织培养与无性快繁体系。[方法]以长春花种子为外植体,分别用1.1%有效氯的次氯酸钠溶液和0.1%HgC l2溶液对长春花种子进行消毒,以MS为基本培养基,通过添加不同浓度的6-BA、IBA和NAA,对脱毒后的长春花试管苗进行增殖和生根培养试验,确定长春花种子的最佳消毒时间,筛选长春花快繁的最佳培养基。[结果]用1.1%有效氯的次氯酸钠消毒长春花种子的最佳消毒时间是30 m in,种子发芽率达96.7%;最适宜的增殖培养基为MS+0.40 mg/L 6-BA+0.05 mg/L IBA,外植体的增殖数最多,且长势旺;诱导生根的最佳培养基为1/2MS+0.10 mg/L IBA+0.10 mg/L NAA,试管苗生根率达100%。[结论]次氯酸钠是长春花种子最好的消毒剂,最适宜的增殖培养基和生根培养基分别为MS+0.40 mg/L 6-BA+0.05 mg/L IBA和1/2MS+0.10 mg/L IBA+0.10 mg/L NAA。
译  名：
Rapid Propagation Techniques of Catharanthus roseus(L.) G.Don
作  者：
CHEN Quan et al(College of Agronomy,Hainan University,Institute of Tropical Biotechnology,Chinese Academy of Tropical Agricultural Science,Haikou,Hainan 571101)
关键词：
Catharanthus roseus(L.) G.Don;Seed disinfection;Proliferation and growth;Root inductio
摘  要：
[Objective] The study aimed to establish the efficient tissue culture and asexual propagation system of Catharanthus roseus(L.) G.Don.[Method] With seeds of C.roseus as explants,they were disinfected with sodium hypochlorite solution containing 1.1% available chlorine and 0.1% mercuric chloride solution,resp..MS was used as the basic medium with different concn.of 6-BA,IBA and NAA to configure different media.The tests of proliferation and rooting culture on the virus-free test-tube plantlets of C.roseus were carried out to determine the best disinfection time of C.roseus seeds and screen the optimal medium for rapid propagation of C.roseus.[Result] The best disinfection time by using sodium hypochlorite solution containing 1.1% available chlorine was 30 min and the seed germination rate was 96.7%.The best proliferation medium for C.roseus was MS+0.40 mg/L 6-BA +0.05 mg/L IBA,on which the explants got more proliferated number and strong growth vigor.The best rooting culture medium for C.roseus was 1/2MS+0.10 mg/L IBA +0.10 mg/L NAA,on which the test-tube plantlets got rooting rate of 100%.[Conclusion] Sodium hypochlorite was the best disinfectant for C.roseus seeds,and the best medium for the proliferation and rooting was MS+0.40 mg/L 6-BA +0.05 mg/L IBA and 1/2MS+0.10 mg/L IBA +0.10 mg/L NAA,resp.

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