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Position: Home > Articles > The polymorphism distribution of coat color gene KIT in Junmu No.1 white pigs,durocs,tibet mini-pigs and large white Chinese Journal of Veterinary Science 2014,34 (12) 2027-2030

军牧1号白猪、杜洛克猪、西藏小型猪和大白猪KIT毛色基因的基因型分析

作  者:
印崇;高峰;李傲楠;张成才;周梦娇;白春艳;孙博兴;赵志辉;邢沈阳
单  位:
吉林大学动物科学学院
关键词:
KIT基因;毛色;猪;PCR-RFLP
摘  要:
毛色是猪的一个重要形态特征,KIT基因是控制猪毛色的主效基因。本研究利用PCR和PCR-SSCP方法,分析了军牧1号白猪、杜洛克猪、西藏小型猪和大白猪的KIT基因拷贝数和第17、18内含子突变位点的基因型,结果显示:军牧1号白猪和大白猪均为多拷贝,而西藏小型猪和杜洛克猪的KIT基因则为单拷贝;军牧1号白猪和大白猪的第17、18内含子突变位点上均检测到突变,而杜洛克猪和西藏小型猪均没有检测到突变。
译  名:
The polymorphism distribution of coat color gene KIT in Junmu No.1 white pigs,durocs,tibet mini-pigs and large white
作  者:
YIN Chong;GAO Feng;LI Ao-nan;ZHANG Cheng-cai;ZHOU Meng-jiao;BAI Chun-yan;SUN Bo-xing;ZHAO Zhi-hui;XING Shen-yang;College of Animal Science,Jilin University;
关键词:
KIT gene;;coat color;;swine;;PCR-SSCP
摘  要:
Coat color is an important character of swine.KIT gene is a major gene that controls coat color of swines.The PCR and PCR-SSCP technique were used to analysis KIT gene copy number and genotypes of intro 17,18 mutation site on Junmu No.1white pigs,durocs,tibet mini-pigs and large white.Result showed that both Junmu No.1white pig and large white are multi-copied,while durocs and tibet mini-pigs are single copied.Mutation was detected on intro 17 and 18mutation site of Junmu No.1and large white,but not found in duroc and tibet mini-pigs.

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