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Position: Home > Articles > The Quantitative Determination of Glycyrrhetinic Acid in Powdered Extracted Glycyrrhizin by RP-HPLC Journal of Tarim University 2006,18 (3) 34-35+82

RP-HPLC法测定甘草浸膏中甘草次酸含量

作  者:
吴瑛;杨玲;卢士林
单  位:
新农开发股份有限公司;塔里木大学文理学院
关键词:
甘草浸膏;甘草次酸;反相高效液相色谱
摘  要:
方法采用反相高效液相色谱法,以甲醇80%和水20%(用磷酸调PH为3.7)为流动相,以C8为分析柱,紫外检测波长为250nm,柱温室温,结果线性范围:0.01mg/m l~0.13mg/m l,线性方程A=50.535+31130.2C,相关系数0.99989;回收率101.85%,相对标准偏差1.47%。该方法目的在于测定甘草浸膏的甘草次酸含量,以期为检测评价甘草浸膏质量提供了指标,该方法方法简单、准确性高。
译  名:
The Quantitative Determination of Glycyrrhetinic Acid in Powdered Extracted Glycyrrhizin by RP-HPLC
作  者:
Wu Ying~1 Yang Lin~1 Lu Shiling~2(1 College of Arts and Science,Tarim University,Alar Xinjiang 843300) (2 Xinnong Development Limited,Alar Xinjiang 843300)
关键词:
powdered extracted glycyrrhizae;Glycyrrhizic acid;reversed phase high performance liquid chromatography(RP-HPLC)
摘  要:
Aiming To develop a method to determine quantitatively the contents of glycyrrhetinic acid in Powdered Extractor Glycyrrhizae by RP-HPLC.RP-HPLC was used to quantitatively determine the contents of glycyrrhetinic acid.During experiments,the detection wavelength was 250nm room temperature.Results were good liner relations and the relative peak area of glycyrrhetinic acid r=0.99989.The average recovery of glycyrrhetinic acid was 101.85%,RSD1.47%.Conclusion: The method was simple,accurate and quick.

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