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当前位置: 首页 > 文章 > 采收期对阿克苏地区灰枣cAMP含量的影响研究 安徽农业科学 2015 (16) 291-293+314
Position: Home > Articles > Influence of Harvest Time of Huizao Jujube on Their Content of c AMP in Aksu Area Journal of Anhui Agricultural Sciences 2015 (16) 291-293+314

采收期对阿克苏地区灰枣cAMP含量的影响研究

作  者：
史洪君;刘琦;孟潇;向延菊
单  位：
塔里木大学生命科学学院/南疆特色农产品深加工兵团重点实验室
关键词：
灰枣;采收时间;环磷酸腺苷;HPLC
摘  要：
[目的]研究测定灰枣c AMP含量的新方法及灰枣采收期对其c AMP含量的影响。[方法]以阿克苏地区灰枣为研究对象,建立了HPLC法测定灰枣中c AMP含量的定量测定方法,并对不同采收时期的灰枣及其制干产品的c AMP含量进行了测定。[结果]试验表明,HPLC测定c AMP含量的色谱条件为:选用Inertsil ODS-3色谱柱(4.6×250×5μm)、以10%甲醇-90%的浓度为0.167 8%甲酸溶液为流动相、柱温30℃、流速0.8 ml/min、检测波长254 nm测定灰枣中的c AMP含量,c AMP浓度在1.0~100.0μg/ml范围内与峰面积具有良好的线性关系,R2=0.999 0,且其精密性高、重现性好、加标回收率高(>98%,RSD值为1.38%)。灰枣采收时期对鲜枣及其制干产品的c AMP含量有显著影响(达到0.01显著水平)。[结论]阿克苏地区灰枣在10月24日至10月31日期间采收,有利于保持较高的c AMP含量,此期采收的鲜灰枣及其干枣的c AMP含量分别为75.237和294.097μg/g左右。研究可为新疆阿克苏地区红枣产业化发展提供科学参考。
译  名：
Influence of Harvest Time of Huizao Jujube on Their Content of c AMP in Aksu Area
作  者：
SHI Hong-jun;LIU Qi;MENG Xiao;XIANG Yan-ju;College of Life Science,Tarim University/ Xinjiang Production & Construction Corp Key Laboratory for Advanced Processing of Local Agricultural Products in Southern Xinjiang;
关键词：
Zizyphus jujube cv.Huizao;;Harvest time;;Adenosine cyclic 3,5-monophosphate;;HPLC
摘  要：
[Objective] To develop new method for determination of c AMP content in Huizao jujube,and study effects of harvest time on c AMP content. [Method] With Huizao jujube in Aksu area as research object,the HPLC method was established for determination of c AMP content,c AMP content in Huizao jujube and its dried products in different harvest time was determined. [Result] The results as follows: the appropriate method with HPLC,were using Inertsil ODS-3 column( 4. 6 × 250 × 5 μm) at 30 ℃ as stationary phase,using solution composed of methanol-0. 167 8% formic acid( 10∶ 90) as mobile phase,detection wavelength at 254 nm,and flow rate with 0. 8 ml / min. There was a good linear relationship between the concentration of c AMP and area integral value in the range of 1. 0- 100. 0 μg / m,R2= 0. 999 0. The average recovery was over 98. 0% with RSD = 1. 38. There was significant impact at 0. 01 significant level between harvest time of Huizao jujube and their content of c AMP in fresh and dry fruits in Aksu Area. [Conclusion] The appropriate harvest time was on between October 24 and 31 to obtain higher content of c AMP in Aksu Area,which was 75. 237,294. 097 μg / g or so respectively. The study can provide scientific reference for Huizao jujube industrial development in Aksu Area.

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