当前位置: 首页 > 文章 > NaCl胁迫下星星草生理响应及分子机制的研究 北方园艺 2010 (7) 31-33
Position: Home > Articles > Physiological Response and Molecular Mechanism of Puccinellia tenuiflora under NaCl Stress Northern Horticulture 2010 (7) 31-33

NaCl胁迫下星星草生理响应及分子机制的研究

作  者:
王雷;吴丽丽;曲跃军;吕澈妍;郑威;姜廷波
单  位:
东北林业大学林木遗传育种与生物技术教育部重点实验室
关键词:
星星草;NaCl胁迫;生理响应;基因表达
摘  要:
研究了星星草在NaCl持续胁迫过程中POD、SOD、MDA、Pro的变化情况。结果表明:SOD活性随着胁迫时间的延长呈现先逐渐增强后逐渐减弱的趋势,峰值出现在持续胁迫的第6天。而POD活性则呈现平稳增长的趋势,但在胁迫处理的第10天骤然增加。在NaCl胁迫过程中,Pro含量增加;MDA含量除在持续胁迫的第4天高于对照外,其余胁迫时间变化并不明显。利用Real-ti me PCR方法研究了NaCl胁迫下CAT、DHAR、TRX基因在转录水平上的表达情况,结果显示3种基因的表达量均高于对照,有效降低了活性氧对星星草的伤害。
译  名:
Physiological Response and Molecular Mechanism of Puccinellia tenuiflora under NaCl Stress
作  者:
WANG Lei,WU Li-li,QU Yue-jun,LV Che-yan,ZHENG Wei,JIANG Ting-bo (Key Laboratory of Forest Tree Genetic Improvement and Biotechnology of Ministry of Education,Northest Forest University,Harbin,Heilongjiang 150040)
关键词:
Puccinellia tenuiflora;NaCl stress;physiological response;gene expression
摘  要:
The changes of POD,SOD,MDA and Pro in Puccinellia tenuiflora were investigated under NaCl stress.The results showed that SOD activity was enhanced gradually,and then weakened.The peak value of SOD was appeared at the 6th day under NaCl stress.The POD activity showed a steady growth trend,but increased rapidly at the 10th day.The proline content was higher than the control.Besides that MDA content at the 4th day was higher than control,MDA content was no significant differences with control during other days.Real-Time PCR demonstrated that the transcript level of CAT,DHAR,TRX was higher than the control.The expression of these genes played an important role in preventing ROS harm to Puccinellia tenuiflora.

相似文章

计量
文章访问数: 6
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊