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Position: Home > Articles > Optimization of Enzymatic Preparation Conditions of Functional Polypeptide from Male Silkworm Moth Protein FOOD SCIENCE 2008,29 (12) 417-421

雄蚕蛾蛋白制备功能性多肽酶解条件优化研究

作  者:
张鹭;唐道邦;肖更生;刘吉平;徐玉娟;邹宇晓
单  位:
华南农业大学动物科学学院;广东省农业科学院蚕业与农产品加工研究所
关键词:
雄蚕蛾;功能性多肽;碱性蛋白酶;二次回归正交旋转组合设计
摘  要:
以水解度(DH)和肽得率为指标,分别考察温度、pH值、底物浓度、加酶量对碱性蛋白酶酶解雄蚕蛾蛋白制备功能性多肽的影响,通过二次回归正交旋转组合设计确定碱性蛋白酶酶解反应的最佳优化参数为:温度64.5℃、pH9.36、底物浓度4.46%、加酶量6660U/ml、反应时间300min。在此条件下,酶解液水解度达到21.65%、肽得率达到34.81%。
译  名:
Optimization of Enzymatic Preparation Conditions of Functional Polypeptide from Male Silkworm Moth Protein
作  者:
ZHANG Lu1,2,TANG Dao-bang1,XIAO Geng-sheng1,,LIU Ji-ping2,XU Yu-juan1,ZOU Yu-xiao1(1. Sericulture and Farm Product Processing Research Institute,Guangdong Academy of Agricultural Science,Guangdong Open Access Laboratory of Agricultural Product Processing,Guangzhou 510610,China;2. School of Animal Science,South China Agricultural University,Guangzhou 510642,China)
关键词:
male silkworm moth;functional polypeptides;alkaline protease;orthogonal rotation combination design
摘  要:
Taking hydrolysis degree and peptide yield as an indexes,the effect of temperature,pH,substrate concentration,alkaline amount on enzymolysis of male silkworm moth protein were studied. The optimal conditions are determined by orthogonal rotation combination design as follows:64.5 ℃,pH 9.36,substrate concentration 4.46%,protease amount 6660 U/ml;and reaction time 300 min. Under these conditions,the hydrolysis degree is up to 21.65% and the peptide yield is up to 34.81%.

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