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六种金属离子对轮梗霉生长及产花生四烯酸的影响

作  者:
王安琪;戴传超;陈佳昕;姚俊杰
单  位:
南京师范大学生命科学学院微生物工程重点实验室
关键词:
花生四烯酸;生长;轮梗霉;金属离子
摘  要:
研究工业发酵中常见的Ca2+、Zn2+、Mn2+、Fe3+、Cu2+及Co2+对轮梗霉生长及产花生四烯酸的影响。结果表明:Ca2+浓度在10-4~10-2mol/L范围内促进菌体生长,但抑制AA产量。Mn2+浓度为5×10-4mol/L时AA产量达到最大,但对菌体生长都有抑制作用。Zn2+在3×10-6mol/L浓度时AA产量达最大,而生物量的变化不大。Fe3+浓度为5×10-5mol/L时,生物量达最大,而AA产量受其抑制,Cu2+浓度为5×10-6mol/L时菌体生物量和AA产量同时达到最大。Co2+在10-7mol/L浓度时生物量及AA产量同时达最大。
译  名:
Effects of Metal Ions Addition on the Growth of and Arachidonic Acid Production by Diasporangium sp.
作  者:
WANG An-qi,DAI Chuan-chao*,CHEN Jia-xin,YAO Jun-jie (Key Laboratory of Microbial Engineering, College of Life Science, Nanjing Normal University, Nanjing 210097, China)
关键词:
Arachidonic acid;growth;Diasporangium sp. ;metal ions
摘  要:
The culture conditions for arachidonic acid(AA) production and the growth by Diasporangium sp.were investigated. Ca2+ or Fe3+ enhanced the cell growth, but suppressed the AA production, while Mn2+ revealed the possibility of the enhancement on cell growth but suppression of AA production. With the addition of Zn2+, AA yield reached higher, but there was little effect on cell growth. A medium containing 5×10-6mol/L Cu2+ or 10-7mol/L Co2+ was found to be the optimum for the AA yield and cell growth.

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