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Position: Home > Articles > Genetic Diversity Analysis on Chickpea Based on ISSR Markers Chinese Journal of Grassland 2015,37 (3) 19-25

基于ISSR分子标记的鹰嘴豆遗传多样性分析

作  者:
靳晓丽;田新会;杜文华
单  位:
甘肃农业大学草业学院/草业生态系统教育部重点实验室/中-美草地畜牧业可持续研究中心
关键词:
鹰嘴豆;遗传多样性;ISSR分子标记
摘  要:
以来自印度和澳大利亚的16份鹰嘴豆种质为试验材料,应用ISSR分子标记对其进行遗传多样性分析,为今后鹰嘴豆育种亲本的筛选提供参考。结果表明,7条ISSR引物对所有鹰嘴豆材料进行扩增,共获得60条带,其中多态性条带数为44条(76.05%)。遗传相似系数(GS)变化范围为0.5806~0.9545,平均遗传相似系数为0.8182。通过聚类分析,在GS=0.83处可把16份鹰嘴豆种质聚为5类,其中ICCV05111(第Ⅰ类)、FLIP97-114C(第Ⅳ类)和Jimbour(第Ⅴ类)分别单独聚为一类,ICCV98818、ICCV98816和ICCV06107聚为一类(第Ⅱ类),其他10份鹰嘴豆材料聚为一类(第Ⅲ类)。
译  名:
Genetic Diversity Analysis on Chickpea Based on ISSR Markers
作  者:
JIN Xiao-li;TIAN Xin-hui;DU Wen-hua;Pratacultural College,Gansu Agricultural University;Key Laboratory of Grassland Ecosystem,Ministry of Education;Sino-U.S.Centers for Sustainable Development of Grassland and Animal Husbandry;
关键词:
Chickpea;;Genetic diversity;;ISSR markers
摘  要:
16chickpea genotypes introduced from Australia and India were analyzed for genetic diversity by using inter-simple sequence repeats(ISSR)markers in the study.The results showed that all chickpea materials were amplified with seven ISSR primers and 60 DNA fragments were amplified,among which there were 44(76.05%)polymorphic DNA bands.Genetic similarity coefficient varies between0.5806~0.9545 and the average was 0.8182.Cluster analysis showed that 16 chickpea genotypes could be divided into 5groups at the GS=0.83.ICCV05111(GroupⅠ),FLIP97-114C(Group Ⅳ)and Jimbour(GroupⅤ)were clustered into a separate group,respectively.ICCV98818,ICCV98816 and ICCV06107were clustered into Group Ⅱ,and the other 10 chickpea genotypes were clustered into Group Ⅲ.

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