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当前位置: 首页 > 文章 > miRNA-145、Oct4和Sox2基因在小鼠早期胚胎发育分化中的表达情况 南方农业学报 2013,44 (12) 2075-2079
Position: Home > Articles > Expression of miRNA-145,Oct4 and Sox2 in early embryonic development and differentiation in mouse Journal of Southern Agriculture 2013,44 (12) 2075-2079

miRNA-145、Oct4和Sox2基因在小鼠早期胚胎发育分化中的表达情况

作  者：
王塑天;李敏霞;娄娟;左二伟;陆阳清;卢克焕;杨小淦
单  位：
广西大学动物科学技术学院
关键词：
小鼠;早期胚胎;发育分化;miRNA-145;Oct4;Sox2
摘  要：
【目的】揭示miRNA-145、Oct4和Sox2基因与早期胚胎细胞表型间可能存在的关联,为阐明miRNA-145调控早期胚胎发育分化的分子机制奠定基础。【方法】利用手术法收集SPF级昆明小白鼠体内生产的2-细胞胚胎和囊胚,采用SYBR Green I实时荧光定量PCR检测小鼠2-细胞胚胎和囊胚中miRNA-145、Oct4和Sox2基因的表达情况。【结果】在小鼠早期胚胎由2-细胞胚胎发育至囊胚的过程中,miRNA-145在囊胚中的表达量显著高于在2-细胞胚胎中的表达量(P<0.05,下同),而Oct4和Sox2在囊胚中的表达量显著低于在2-细胞胚胎中的表达量。【结论】miRNA-145基因表达量上调与Oct4、Sox2基因表达量下调是随着小鼠早期胚胎发育分化的进行同时发生。
译  名：
Expression of miRNA-145,Oct4 and Sox2 in early embryonic development and differentiation in mouse
作  者：
WANG Su-tian;LI Min-xia;LOU Juan;ZUO Er-wei;LU Yang-qing;LU Ke-huan;YANG Xiao-gan;College of Animal Science and Technology,Guangxi University;State Key Laboratory of Subtropical Bio-resources Conservation and Utilization;
关键词：
mouse;;early embryo;;development and differentiation;;miRNA-145;;Oct4;;Sox2
摘  要：
【Objective】The probable relation among miRNA-145, Oct4, Sox2 and the phenotype of early embryo was revealed to provide references for exploring the molecular mechanism of miRNA-145 in mouse early embryonic differentiation. 【Method】Two-cell embryos and blastocysts of KM mouse produced in vivo were collected by surgical method. The expression of miRNA-145, Oct4 and Sox2 in two-cell embryos and blastocysts was detected by real-time PCR. 【Result】During the development of early embryo in mouse from two-cell embryos to blastocysts, the expression of miRNA-145 in blastocysts was significantly higher than that in two-cell embryos(P<0.05), and the expression of Oct4 and Sox2 in blastocysts was both significantly lower than that in two-cell embryos(P<0.05). 【Conclusion】During the development of early embryo in mouse, the upregulated expression of miRNA-145 and reduced expression of Oct4, Sox2 and the early embryonic differentiation happened simultaneously.

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