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Position: Home > Articles > Purification and Characterization of Bacillus subtilis Nattokinase FOOD SCIENCE 2012,33 (15) 231-234

枯草芽孢杆菌纳豆激酶分离纯化及酶学性质

作  者:
王刚;郭明珠;陈光
单  位:
吉林农业大学生命科学学院;长春职业技术学院
关键词:
纳豆激酶;枯草芽孢杆菌;纯化;酶学特性
摘  要:
利用30%~70%饱和度的硫酸铵沉淀,Sephadex G-50凝胶过滤层析对浅盘发酵纳豆激酶进行分离纯化并对其酶学性质进行初步研究。以纤维平板法测定纳豆激酶活力,SDS-PAGE检验纯化效果。结果表明:纯化后纳豆激酶为电泳纯,分子质量约27.518kD,纯化倍数和酶活回收率分别为19和42.1%,纳豆激酶最适温度为50℃,最适宜pH值为8.0。
译  名:
Purification and Characterization of Bacillus subtilis Nattokinase
作  者:
WANG Gang 1,GUO Ming-zhu 2,CHEN Guang 1,(1.College of Life Science,Jilin Agricultural University,Changchun 130118,China;2.Changchun Vocational Institute of Technology,Changchun 130033,China)
关键词:
nattokinase;Bacillus subtilis;purification;characterization
摘  要:
In this study,nattokinase produced by Bacillus subtilis NK-1 after shallow tray fermentation was separated and purified by 30%-70% saturation ammonium sulfate precipitation and Sephadex G-50 column chromatography and enzymatically characterized.Nattokinase activity was measured by fibrin plate method,and SDS-PAGE was used to analyze its purity.Electrophoresis-pure nattokinase was obtained,with a molecular weight of about 27.518kD.The purification factor and activity recovery were 19 and 42.1%,respectively.The optimal reaction temperature and pH of this enzyme were 50 ℃ and 8.0,respectively.

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