当前位置: 首页 > 文章 > 胃蛋白酶提取硫酸软骨素工艺优化 肉类研究 2011,25 (8) 22-25
Position: Home > Articles > Optimization of Chondroitin Sulfate Extraction from Bovine Pipes by Ultrasonic-assisted dual-enzyme Hydrolysis MEAT RESEARCH 2011,25 (8) 22-25

胃蛋白酶提取硫酸软骨素工艺优化

作  者:
田甲春;余群力
单  位:
甘肃农业大学食品科学与工程学院
关键词:
硫酸软骨素;胃蛋白酶;得率;纯度
摘  要:
以牛喉管为原料,采用超声波辅助碱-双酶(木瓜蛋白酶、胃蛋白酶)法提取硫酸软骨素。通过单因素试验、正交试验优化胃蛋白酶酶解硫酸软骨素的工艺。结果显示:当pH值为5.9、胃蛋白酶添加量为1.4:1000(m/V)、酶解温度40℃、酶解时间为100min时,产品最高得率可达25.43%,纯度达到87.96%。
译  名:
Optimization of Chondroitin Sulfate Extraction from Bovine Pipes by Ultrasonic-assisted dual-enzyme Hydrolysis
作  者:
TIAN Jia-chun,YU Qun-li(College of Food Science and Engineering,Gansu Agricultural University,Lanzhou 730070,China)
关键词:
chondroitin sulfate;pepsin;yield;purity
摘  要:
Ultrasonic pretreatment followed by sequential hydrolysis with papain and pepsin was used to extract chondroitin sulfate from bovine pipes.The optimal pepsin hydrolysis conditions were identified by one-factor-at-a-time and orthogonal array design methods to be pH 5.9,pepsin dosage 1.4:1000(m/V),temperature 40 ℃ and hydrolysis time 100 min.Under these conditions,the extraction yield of chondroitin sulfate was 25.43% with a purity of 87.96%.All quality indicators investigated completely reached the requirements of relevant national standards for chondroitin sulfate.

相似文章

计量
文章访问数: 12
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊