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当前位置: 首页 > 文章 > AP-1及其siRNA对心肌细胞中TGF-β_1的影响 动物医学进展 2016,37 (12) 46-49
Position: Home > Articles > Effects of AP-1 and siRNA on TGF-β_1 in Myocardial Cells Progress in Veterinary Medicine 2016,37 (12) 46-49

AP-1及其siRNA对心肌细胞中TGF-β_1的影响

作  者：
李斌;陈晶
单  位：
包头医学院基础医学与法医学院
关键词：
AP-1;TGF-β1;心肌细胞;RNA干扰;实时荧光定量PCR
摘  要：
采用RNA干扰方法研究转录因子AP-1对TGF-β1的转录调控影响及其对心肌细胞的影响。设计合成两对AP-1基因的siRNA-59和siRNA-60,同时合成与AP-1基因序列无关的siRNA-58,试验设置阴性对照、阳性对照组及干扰组(AP-1-58、AP-1-59和AP-1-60)共5组,使用LipofectantineTM2000转染AP-1siRNA入培养的大鼠心肌细胞,倒置显微镜观察细胞形态;采用实时荧光定量PCR法检测AP-1和TGF-β1在心肌细胞中mRNA水平表达量的变化。结果显示,siRNA-58对AP-1的基因沉默(表达下调)无显著差异(P>0.05),siRNA-59和siRNA-60对AP-1的基因沉默(表达下调)有显著差异(P<0.05),同时对应siRNA-59和siRNA-60组的TGF-β1基因有显著性下调(P<0.05),说明AP-1和TGF-β1的基因表达存在正相关。
译  名：
Effects of AP-1 and siRNA on TGF-β_1 in Myocardial Cells
作  者：
LI Bin;CHEN Jing;Department of Basic Medicine and Forensic Medicine,Baotou Medical College;
关键词：
activator protein-1;;transforming growth factorβ1;;cardiomyocyte;;RNA interference;;realtime PCR
摘  要：
To study the effect of transcription factor AP-1on TGF-β1and cardiomyocytes by using RNA interference method,two pairs of siRNA of AP-1genes were designed and synthesized,meanwhile unrelated siRNA of AP-1genes was synthesized as control.The experiment was divided into five groups,including negative control group,positive control group and three interference groups(siRNA-58,siRNA-59,siRNA-60).The cultured rat cardiomyocytes were transfected with AP-1siRNA using LipofectamineTM2000.Cell morphology was observed by inverted microscope.The changes of mRNA expressions of AP-1and TGF-β1in cardiomyocytes were detected by real-time quantitative PCR.The results showed,there was no significant difference in the expression of AP-1gene in siRNA-58 group.In the siRNA-59 group and siRNA-60 group,the expression of AP-1gene decreased significantly,while the expression of TGF-β1gene was significantly reduced.The results indicated that the expression of AP-1gene and TGF-β1gene were positively correlated.

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