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Position: Home > Articles > Isolation and 16 S rDNA Identification of E.coli Strains from Geese Infected with Yolk-sac Peritonitis China Poultry 2011,33 (14) 29-31+35

致鹅卵黄性腹膜炎大肠杆菌的分离与16SrDNA的鉴定

作  者:
钱琨;张永志;金文杰;邵红霞;秦爱建
单  位:
扬州大学兽医学院江苏省动物预防医学重点实验室禽类预防医学省部共建教育部重点实验室
关键词:
鹅卵黄性腹膜炎;大肠杆菌;16SrDNA;分离鉴定
摘  要:
从鹅卵黄性腹膜炎临床病例中采集样品,分离病原进行一系列生化鉴定,共分离得到7株致鹅卵黄性腹膜炎大肠杆菌,编号为E0238、E0239、E0240、E0453、E0454、E0241、E0245。O血清型鉴定分属于O2、O21、O37、O1、O24、O24、O148。利用16SrDNA扩增试剂盒对随机选取的3株细菌进行PCR扩增,并将PCR产物序列与GenBank中的E.coli16SrDNA进行序列比对,结果显示分离细菌与大肠杆菌的同源性达99%以上。
译  名:
Isolation and 16 S rDNA Identification of E.coli Strains from Geese Infected with Yolk-sac Peritonitis
作  者:
QIAN Kun,ZHANG Yongzhi,JIN Wenjie,SHAO Hongxia,QIN Aijian**(Key Lab for Avian Preventive Medicine of Ministry of Education & Key Lab of Jiangsu Preventive Veterinary Medicine,College of Veterinary Medicine,Yangzhou University,Yangzhou,Jiangsu 225009)
关键词:
geese yolk-sac peritonitis;E.coli;16 S rDNA;isolation and identification
摘  要:
Samples from dead geese infected with yolk-sac peritonitis were collected from the veterinary stations.Seven bacteria strains were isolated and identified by biochemistry tests,which named as E0238,E0239,E0240,E0453,E0454,E0241 and E0245.The O antigen test results showed that O serotypes of these strains were O2,O21,O37,O1,O24,O24 and O148 respectively.Three random selected strains were detected by polymerase chain reaction(PCR) and compared with 16 S rDNA sequence of Escherichina coli(E.coli) from GenBank.The sequence analysis revealed that the homology could exceed 99%,which confirmed the isolated bacteria were E.coli.

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