当前位置: 首页 > 文章 > 白花草木樨EST-SSR标记的开发与筛选 草业科学 2017 (9) 1802-1814
Position: Home > Articles > The development and screening of EST-SSR markers in Melilotus albus Pratacultural Science 2017 (9) 1802-1814

白花草木樨EST-SSR标记的开发与筛选

作  者:
剡转转;任艳;吴凡;骆凯;张代玉;闫启;张宇飞;赵玉凤;张吉宇
单  位:
草地农业生态系统国家重点实验室兰州大学草地农业科技学院
关键词:
草木樨;EST-SSR;标记开发;多态信息含量;期望杂合度;遗传距离;遗传多样性
摘  要:
草木樨(Melilotus)是重要的豆科牧草之一,然而草木樨分子标记匮乏,限制了草木樨种质资源的开发与利用。本研究以白花草木樨(M.albus)转录组数据为基础,设计了18 182对草木樨EST-SSR引物,并对所开发的EST-SSR引物进行筛选。通过PCR扩增从550对EST-SSR引物中筛选得到206对白花草木樨多态性引物,共检测出679个等位基因,平均每对引物检测出2.888个基因位点。多态信息含量PIC的分布范围为0.239~0.855,平均值为0.468。206对多态性引物Nei’s基因多样性指数(H)和Shannon信息指数(I)的平均值分别为0.169和0.239。本研究开发的EST-SSR丰富了草木樨属的分子标记,为研究种质资源的遗传多样性和分子辅助育种奠定了基础。
译  名:
The development and screening of EST-SSR markers in Melilotus albus
作  者:
Yan Zhuan-zhuan;Ren Yan;Wu Fan;Luo Kai;Zhang Dai-yu;Yan Qi;Zhang Yu-fei;Zhao Yu-feng;Zhang Ji-yu;State Key Laborotary of Grassland Agro-ecosystems,College of Pastoral Agriculture Science and Technology,Lanzhou University;
关键词:
Melilotus;;EST-SSR;;marker development;;polymorphism information content;;expected heterozygosity;;genetic distance;;genetic diversity
摘  要:
Melilotus is one of the most important legume forages,however,the lack of molecular markers has limited the development and utilization of Melilotus germplasm resources in breeding programs.In this study,18 182EST-SSR primers were developed and screened based on transcriptome data from M.albus.A total of206 primer pairs from M.albus were found to be polymorphic from a total of 550 pairs of EST-SSR primers screened.A total of 679 alleles were detected with an average of 2.888 alleles per locus.The polymorphism information content(PIC)values ranged from 0.239 to 0.855,with an average of 0.468.Nei's gene diversity and Shannon's information index for 206 pairs of polymorphic primers were 0.169,and 0.239,respectively.The EST-SSRs developed in this study enriches the EST-SSR molecular markers of Melilotus,which will serve as the foundation for exploring the genetic diversity of germplasm resources for marker-assisted breeding.

相似文章

计量
文章访问数: 8
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊