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Position: Home > Articles > Colorimetric Determination of Soyasaponins Based on Soyasapogenol Moiety FOOD SCIENCE 2009,30 (2) 211-214

苷元比色法测定大豆总皂苷

作  者:
师文添;于学雷;袁建;赵大云
单  位:
上海交通大学食品科学与工程系;南京财经大学食品科学与工程学院
关键词:
大豆苷元;大豆皂苷;比色法
摘  要:
本实验以大豆胚芽为原料,香草醛-高氯酸为显色剂、冰醋酸为溶剂,大豆皂苷Bb为标准品,通过大豆皂苷苷元显色,在波长563nm处测定吸光度,建立了苷元比色法测定大豆总皂苷的定量方法。实验证实该方法线性和稳定性可靠。
译  名:
Colorimetric Determination of Soyasaponins Based on Soyasapogenol Moiety
作  者:
SHI Wen-tian1,YU Xue-lei1,YUAN Jian2,ZHAO Da-yun1,(1. Department of Food Science and Technology,Shanghai Jiao Tong University,Shanghai 200240,China; 2. College of Food Science and Engineering,Nanjing University of Finances and Economics,Nanjing 210003,China)
关键词:
soyasapogenol;soyasaponin;colorimetric determination
摘  要:
One major triterpenoid saponins were isolated from soybean hypocotyls and identified as soyasaponin Bb. The prepared soyasaponin Bb was used as authentic standard for colorimetric determination of soyasaponin content based on the aglycone moiety (soyasapogenol). The employed coloring reagent was vanillin-acetic acid and the determination was made at the wavelength of 563 nm. The results showed that the developed colorimetric assay method is simple,sensitive and relatively precise.

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