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当前位置: 首页 > 文章 > 家蚕5龄幼虫期乙酰化修饰丝胶蛋白对蚕丝性能的影响 蚕业科学 2016 (4) 688-692
Position: Home > Articles > Effect of Acetylation of Sericin at the 5th Instar on the Physical Characteristics of Bombyx mori Silk Science of Sericulture 2016 (4) 688-692

家蚕5龄幼虫期乙酰化修饰丝胶蛋白对蚕丝性能的影响

作  者：
聂作明;斯洪强;曹赟洁;周雍;朱红琳
单  位：
浙江理工大学生物化学研究所;浙江理工大学生命科学学院浙江理工大学材料与纺织学院;浙江理工大学生命科学学院
关键词：
蚕丝蛋白;乙酰化修饰;丝胶蛋白;结构;力学性能;5龄幼虫
摘  要：
乙酰化修饰即在翻译中或翻译后的蛋白质分子链上嫁接乙酰基团,是通过化学修饰改变蛋白质性质的方式之一,丝胶蛋白是高度乙酰化的蛋白质。以丝腺组织大量合成丝蛋白的家蚕5龄第2天幼虫供试,分别将去乙酰化酶抑制剂TSA和乙酰化酶抑制剂C646自幼虫第2腹节注射入体内丝腺,上调和下调丝胶蛋白的乙酰化水平,探究通过乙酰化修饰丝胶蛋白的方法改善蚕丝性能的可行性。收集试验组家蚕的茧丝进行力学性能测试、红外光谱分析、X射线衍射分析、扫描电子显微镜(SEM)观察,结果证实丝胶蛋白的乙酰化修饰并不会改变蚕丝丝素的晶体结构,但具有增强丝胶与丝素表层附着力的作用。研究结果可为蚕丝精练脱胶和染整工艺的改进提供新的理论依据。
译  名：
Effect of Acetylation of Sericin at the 5th Instar on the Physical Characteristics of Bombyx mori Silk
作  者：
Nie Zuoming;Si Hongqiang;Cao Yunjie;Zhou Yong;Zhu Honglin;Wu Chengcheng;Jiang Caiying;Sheng Qing;Lyu Zhengbing;Yu Wei;Yao Juming;College of Life Science,Zhejiang Sci-Tech University;College of Materials and Textiles,Zhejiang Sci-Tech University;
关键词：
Silk protein;;Acetylation modification;;Sericin;;Structure;;Mechanical property;;The 5th instar larva
摘  要：
Acetylation modification,in which acetyl group is transferred onto molecular chain of proteins during translation and post-translation,is one of the ways to modify the properties of protein by chemical modification. Sericin is a kind of highly acetylated protein. In present study,day 2 Bombyx mori larvae of the 5th instar,which contain plenty of silk proteins synthesized in silk gland,was used as experimental material to explore the feasibility of improving the performance of silk by acetylation. We firstly injected deacetylase inhibitor TSA and acetylase inhibitor C646 into silk gland from the2 nd abdominal segment to increase and decrease acetylation level of sericin,respectively. Then the acetylated silk was sampled to conduct mechanical property test,IR spectrum analysis,X-ray diffraction analysis and SEM observation. The results suggested that increased acetylation of sericin could not change the crystal structure of silk fibroin,but was helpful to enhance the adhesion of sericins on silk fiber surface. The results could provide a new theoretical basis for technological improvement in degumming,dyeing and finishing of silk.

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