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当前位置: 首页 > 文章 > 贵州柿属植物种质资源遗传多样性的SRAP分析 果树学报 2015,32 (5) 751-762
Position: Home > Articles > Genetic diversity of Diospyros Linn. in Guizhou based on SRAP Journal of Fruit Science 2015,32 (5) 751-762

贵州柿属植物种质资源遗传多样性的SRAP分析

作  者：
韩振诚;潘学军;安华明;张文娥
单  位：
贵州大学·贵州省果树工程技术研究中心
关键词：
柿;SRAP;贵州;遗传多样性
摘  要：
【目的】探明贵州柿种质资源遗传多样性及亲缘关系状况,为其保护与利用提供理论依据。【方法】利用SRAP分子标记技术对贵州83份柿种质资源的遗传多样性及亲缘关系进行分析。【结果】15对SRAP引物一共扩增出118个DNA位点,其中108个位点具有多态性,占总位点的91.53%,等位基因数(Na)为(1.915 3±0.279 7),有效等位基因数(Ne)为(1.418 9±0.352 1),遗传多样性指数Nei’s(He)为(0.252 6±0.176 0),平均Shannon信息指数(I)为(0.390 6±0.234 2),地区间基因流Nm为3.702 6。UPGMA聚类分析表明,相似系数在0.740时83份柿种质资源被分为5组,居群之间的相似系数与地理距离和种质植物学性状间呈一定的相关性。【结论】贵州柿资源存在丰富的多样性,人为因素导致地区间存在较强的基因流,5个地区间柿种质资源遗传多样性依次为:黔中>黔西>黔南>黔北>黔东。SRAP标记可有效揭示贵州柿种质资源的遗传多样性、分化程度和亲缘关系,研究结果对贵州柿种质资源的保护利用和遗传改良有重要意义。
译  名：
Genetic diversity of Diospyros Linn. in Guizhou based on SRAP
作  者：
HAN Zhencheng;PAN Xuejun;AN Huaming;ZHANG Wen'e;Guizhou Engineering Research Center for Fruit Crops,Guizhou University;
关键词：
Diospyros Linn.;;SRAP;;Guizhou province;;Genetic diversity
摘  要：
【Objective】To explore the present situation of Guizhou persimmon germplasm resources genetic diversity and genetic relationship,and providing theoretical basis for its protection and utilization.【Methods】In this study SRAP molecular markers technology was used to analysis the genetic diversity and genetic relationship of 83 Guizhou persimmon germplasm resources.【Results】118 DNA loci were amplified by 15 pairs of SRAP primers,of which 108 loci were polymorphic,accounted for 91.53% of total loci,the number of alleles(Na) was(1.915 3 ± 0.279 7),the effective number of alleles(Ne) was(1.418 9±0.352 1),Nei's gene diversity(He) was(0.252 6 ± 0.176 0),Shannon's information index(I) was(0.390 6±0.234 2). UPGMA cluster analysis showed that the similarity coefficient is about 0.740,the 83 copies of persimmon germplasm resources could be classified into 5 groups,there is a certain correlation between groups of similar coefficient and the distance and germplasm botany traits.【Conclusion】The Guizhou persimmon resources contain abundant genetic diversity,and human factors leading to the presence of strong gene flow among regions. The 5 regions of persimmon germplasm resources genetic diversity were,in order,the Centre > West > Southern > Northern > Eastern. SRAP marker technology can effectively reveal the genetic diversity,the degree of differentiation and phylogenetic relationship of persimmon germplasm resources in Guizhou. And the results also have important significance in protecting,utilizing and genetic improving of persimmon germplasm resources in Guizhou.

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