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Position: Home > Articles > Cloning and Sequence Analysis of a Flowering-related MADS-box Gene in Narcissus tazetta var.chinensis Roem. Acta Horticulturae Sinica 2008,35 (2) 295-300

一个中国水仙MADS-box基因的克隆与分析

作  者:
高志民;陈段芬;李雪平;蔡春菊;彭镇华
单  位:
国际竹藤网络中心国家林业局竹藤科学与技术重点开放实验室;中国林业科学研究院林业研究所
关键词:
中国水仙;MADS-box基因;克隆;系统进化树分析
摘  要:
采用RT-PCR技术从中国水仙(Narcissus tazetta var.chinensis Roem.)幼嫩花蕾中分离到一个新的水仙花发育相关MADS-box基因,命名为NTMADS1(GenBank登录号:EF421828)。该基因长879bp,编码区共编码230个氨基酸,具有典型的植物MADS-box基因结构,由MADS区、I区、K区和C末端组成。序列分析结果表明,NTMADS1编码的蛋白与其它植物的MADS-box蛋白有着较高的一致性,其中与扫状天门冬(Asparagus virgatus Baker)的AVAG1的一致性高达89%。系统进化树分析表明NTMADS1属于AG亚族。
译  名:
Cloning and Sequence Analysis of a Flowering-related MADS-box Gene in Narcissus tazetta var.chinensis Roem.
作  者:
GAO Zhi-min1,CHEN Duan-fen1,LI Xue-ping1,CAI Chun-ju1,and PENG Zhen-hua2(1International Center for Bamboo and Rattan,SFA Key Laboratory of Bamboo and Rattan Science and Technology,Beijing 100102,China;2 Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,China)
关键词:
Narcissus tazetta var.chinensis Roem.;MADS-box gene;clone;phylogenetic tree analysis
摘  要:
MADS-box genes play important role in various plant development processes,especially in the development of floral organs.The flowering-related MADS-box gene,named as NTMADS1(GenBank accession number:EF421828),was cloned from the first strand of Narcissus tazetta var.chinensis Roem.cDNA through RT-PCR using primers designed according to the MADS-box gene family conserved region.The fragment was 879 bp long and contained an open reading frame of 693 bp coding a polypeptide of 230 amino acids which had a typical MADS-box domain.Homology analysis showed that the deduced NTMADS1 protein was highly homologous to other MADS-box proteins from different species,especially with Asparagus virgatus Baker to 89%.Phylogenetic tree analysis also indicated that NTMADS1 belongs to AG subfamily.

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