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当前位置: 首页 > 文章 > L-半胱氨酸聚合膜亚硝酸盐安培传感器的制备及在食品检测中的应用 食品科学 2009,30 (4) 158-161
Position: Home > Articles > A Novel Nitrite Amperometric Sensor Based on Poly(L-Cysteine) Film Electrode and Its Application in Food Analysis FOOD SCIENCE 2009,30 (4) 158-161

L-半胱氨酸聚合膜亚硝酸盐安培传感器的制备及在食品检测中的应用

作  者：
杨春海;刘涛;张升晖
单  位：
湖北民族学院湖北省生物资源保护与利用重点实验室
关键词：
L-半胱氨酸;亚硝酸盐;安培传感器;电聚合
摘  要：
将L-半胱氨酸通过循环伏安法电聚合到玻碳电极表面,制备了一种聚合物薄膜修饰电极,构建了一种新型NO-2安培传感器。实验结果表明,NO-2在该传感器上表现出良好的安培响应。其稳态电流与NO-2的浓度在1.0×10-6～4.4×10-5mol/L范围内呈现良好的线性关系,检测限为1.0×10-7mol/L。此传感器具有灵敏度高、检测限低、抗干扰能力强等优点,应用于食品中NO-2含量的测量,结果满意。
译  名：
A Novel Nitrite Amperometric Sensor Based on Poly(L-Cysteine) Film Electrode and Its Application in Food Analysis
作  者：
YANG Chun-hai,LIU Tao,ZHANG Sheng-hui(Key Laboratory of Biologic Resources Protection and Utilization of Hubei Province,Hubei University for Nationalities,Enshi 445000,China)
关键词：
L-cysteine;nitrite;amperometric sensor;electropolymerization
摘  要：
Glassy carbon electrode surface was modified with L-cysteine by means of cyclic voltammetry to prepare polymer film-modified electrode,and then the electrode was used to construct a novel nitrite amperometric sensor.The electrocatalytic activity of poly(L-cysteine) film electrode toward NO-2 was studied in detail through cyclic voltammetry.A method for determination of nitrite in food using the amperometric sensor was built.The results indicated that the sensor shows an excellent amperometric response to NO-2 in phosphate buffer(PB) at pH 3.0;A good linear relationship over a NO-2 concentration range from 9.0×10-7 mol/L to 1.49×10-5 mol/L is obtained,and the detection limit is 1.0×10-7 mol/L(S/N=3).The sensor shows high sensitivity,good linearity,low detection limit and good selectivity.It was used for quantitative analysis of NO-2 in food samples with satisfying results.

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