当前位置: 首页 > 文章 > 松辽黑猪胰岛素样生长因子Ⅱ基因PCR-SSCP多态性分析 畜牧与兽医 2008,40 (11) 29-31
Position: Home > Articles > PCR-SSCP analysis of IGF-Ⅱ gene in Songliao black pigs Animal Husbandry & Veterinary Medicine 2008,40 (11) 29-31

松辽黑猪胰岛素样生长因子Ⅱ基因PCR-SSCP多态性分析

作  者:
王聪明;张嘉保;赵志辉;张树敏;刘丽丽;袁宝;刘利;刘计峰;岳续朋
单  位:
吉林省农业科学院畜牧分院;吉林大学实验动物中心;吉林大学畜牧兽医学院
关键词:
松辽黑猪;IGF-Ⅱ基因;PCR-SSCP;生长性状
摘  要:
采用PCR-SSCP技术检测胰岛素样生长因子Ⅱ(IGF-Ⅱ)基因外显子3和外显子4在松辽黑猪中的单核苷酸多态性,同时研究该基因对松辽黑猪部分重要生长、胴体性状的影响。结果表明:3对引物中,只有p3的扩增片段存在多态性。p3的扩增片段在松辽黑猪中检测到AA、AB和BB 3种基因型;测序分析发现BB型与AA型相比在IGF-Ⅱ基因(AY044828)第21585 bp处有G→C突变,松辽黑猪AA、AB和BB基因型频率分别为0.6956、0.2609和0.0435。AA基因型对瘦肉率(P<0.05)、日增重(P<0.05),BB基因型对平均背膘厚(P<0.01)有正遗传效应,对其他性状差异不明显。
译  名:
PCR-SSCP analysis of IGF-Ⅱ gene in Songliao black pigs
作  者:
WANG Cong-ming1,ZHANG Jia-bao1,ZHAO Zhi-hui2,ZHANG Shu-min3,LIU Li-li2,YUAN Bao1,LIU Li1,LIU Ji-feng1,YUE Xu-peng1(1.Laboratory Animal Center of Jilin University,Changchun 130062,China;2.College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China;3.Branch of Animal Science Jilin Academy of Agriculture Sciences,Gongzhuling 136100,China)
关键词:
Songliao black pig;insulin-like growthfactor-Ⅱ gene;PCR-SSCP
摘  要:
According to the sequence of IGF-Ⅱ gene,three pairs of primers were designed to detect single nucleotide polymorphisms of exon 3 and exon 4 in Songliao black pigs by PCR-SSCP.The results indicated that only the products amplified by primer p3 displayed polymorphism.Three genotypes(AA,AB and BB) were detected and sequencing analysis revealed a G→C mutation at 21585 bp of IGF-Ⅱ gene.Genotype frequencies of AA,AB and BB were 0.695 6,0.2609 and 0.0435 in Songliao black pigs.The pigs with AA genotype had a higher lean percentage and average daily gain than those with AB and BB genotype(P<0.05),the pigs with BB genotype had a higher average backfat thickness than those with AB and AA genotype(P<0.01).

相似文章

计量
文章访问数: 6
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊