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当前位置: 首页 > 文章 > 1株油茶病害拮抗真菌的鉴定、生物学特性及拮抗作用研究 河南农业科学 2016,45 (8) 74-80
Position: Home > Articles > Identification of Antagonistic Fungus to Camellia Diseases and Study of Biological Characteristics and Antagonism Effect Journal of Henan Agricultural Sciences 2016,45 (8) 74-80

1株油茶病害拮抗真菌的鉴定、生物学特性及拮抗作用研究

作  者：
魏蜜;路露;李春琪;傅本重;李国元;王立华
单  位：
湖北工程学院生命科学技术学院/特色果蔬质量安全控制湖北省重点实验室
关键词：
草酸青霉;鉴定;生物学特性;油茶病害;拮抗作用
摘  要：
为了给油茶病害的生物防治提供科学依据,从油茶根部土壤分离获得1株能拮抗油茶主要病害的真菌F217-3。依据该菌株的形态特征,并结合全自动微生物鉴定系统及r DNA-ITS序列分析,将其鉴定为草酸青霉(Penicilium oxalicum)。对其生物学特性研究结果表明:该菌株在查氏培养基上生长最佳,碳源和氮源分别以蔗糖和尿素最适宜,生长适温为28℃,致死温度为65℃,在p H值为3~10条件下均生长良好,其中最适p H值是4~7,通气与否对菌丝生长无明显影响,但光照24 h条件下菌丝生长较差。平板对峙试验结果表明,F217-3对油茶炭疽病菌(C.nicotianae)、油茶软腐病菌(A.camellia)、黑斑病菌(A.alternate)和溃疡病菌(B.dothidea)的抑菌率均达到90%以上,可抑制油茶胶孢炭疽病菌、黑斑病菌分生孢子产生,抑制或致畸油茶炭疽病菌、溃疡病菌和叶斑病菌菌丝,是一株具有广谱拮抗作用的生防菌株。
译  名：
Identification of Antagonistic Fungus to Camellia Diseases and Study of Biological Characteristics and Antagonism Effect
作  者：
WEI Mi;LU Lu;LI Chunqi;FU Benzhong;LI Guoyuan;WANG Lihua;College of Life Science and Technology,Hubei Engineering University/Hubei Key Laboratory of Quality Control of Characteristic Fruits and Vegetables;
关键词：
Penicilium oxalicum;;identification;;biological characteristics;;Camellia diseases;;antagonism effect
摘  要：
In order to provide scientific basis for the biocontrol of Camellia diseases,a strain of fungus was isolated from soil of Camellia root which could antagonize major Camellia diseases. Following morphological characteristics and quantificational analysis by gas chromatography using the Sherlock microbial identification system( MIDI),combining with the result of the r DNA-ITS sequence analysis,it was identified as P. oxalicum and named P. oxalicum F217-3. The results indicated that F217-3 grew best in CA medium,sucrose and urea were the suitable carbon and nitrogen resource respectively. The strain grew best under 28 ℃,and the lethal temperature was 65 ℃. Although the mycelium could grow at p H 3to 10,p H of 4 to 7 was optimal. Ventilation had no significant effect on the growth of penicillium mycelium,but 24 h light was not beneficial to the mycelial growth. Moreover,the inhibition rates of F217-3 against the mycelia growth of major Camellia pathogens were higher than 90%. It was observed that F217-3 could inhibit the spore germination of C. gloeosporioides and A. alternate,and also inhibit and defect the hyphae of C. nicotianae,B. dothidea and P. capitalensis. The results showed that F217-3 was a biocontrol strain with broad-spectrum antagonism.

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