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Position: Home > Articles > Induction Variation of H_7 Flagellum Antigen and Identification of EHEC O_(157):H_7 from Imported Frozen Chicken Wing FOOD SCIENCE 2004,25 (9) 144-146

出血性大肠杆菌H_7鞭毛抗原的诱导恢复及鉴定

作  者:
王志强;许龙岩;李志勇;凌莉
单  位:
广州出入境检验检疫局
关键词:
出血性大肠杆菌;鞭毛抗原诱导;鉴定
摘  要:
从进口冻鸡翅中分离获得一株发酵山梨醇、缺失H7鞭毛抗原的出血性大肠杆菌O157:H7变异株,采用0.4%强营养软琼脂对该菌进行连续5代诱导培养,使其H7鞭毛抗原成功恢复。综合该菌的生物学特性、血清学及毒理实验结果确证为大肠杆菌O157:H7。该菌的检出为大肠杆菌O157:H7变异株的鉴定提供了有益参考。
译  名:
Induction Variation of H_7 Flagellum Antigen and Identification of EHEC O_(157):H_7 from Imported Frozen Chicken Wing
作  者:
WANG Zhi-qiang,XU Long-yan,LI Zhi-yong,LING Li(Guangzhou Entry-exit Inspection and Quarantine Bureau, Guangzhou 510623, China)
关键词:
EHEC O157:H7;induction of flagellum antigen;identification
摘  要:
A variation EHEC O157:H7 strain was isolated from imported frozen chicken wing, while fermented sorbitol and lackedH7 flagellum antigen, was identified as EHEC O157:H7 by the combination of biological characters, serological result and toxicologicaltest. And the lost H7 flagellum antigen of EHEC O157:H7 was successfully induced after continuous 5-generation induction via0.4% high-nutrition soft agar. Our study was a useful reference for the identification of other variation EHEC O157:H7 strain.

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