关键词：
在线固相萃取富集;牛奶;高效液相色谱法;黄曲霉毒素M_1
摘 要：
目的:建立在线固相萃取富集-高效液相色谱法(On-line SPE-HPLC)测定牛奶中黄曲霉毒素M1。方法:固相萃取富集柱填料为SB-C_(18),上样溶剂和淋洗溶剂均为纯水,转移溶剂为乙腈-水(20:80,V/V),分析测定溶剂为乙腈-水(15:85,V/V)。结果:黄曲霉毒素M1检测的线性方程为Y=2.52746X 0.067,相关系数为0.99906;检出限为0.04μg/kg;定量限为0.10μg/kg;基质4个水平添加回收率(n=6)在98%~105.7%;6次加标回收相对标准偏差在0.79%~2.56%。结论:此方法简便、准确、重现性好,能够满足对日常牛奶中黄曲霉毒素M_1的定量检测。
译 名：
Determination of Aflatoxin M 1 in Milk by High Performance Liquid Chromatography Using On-line Solid Phase Extraction
作 者：
丁俭(1);;李培武(1);;李光明(1);;王秀嫔(1);;马飞(1)
单 位：
(1)中国农业科学院油料作物研究所, 农业部油料作物生物学与遗传育种重点实验室;;;;农业部生物毒素检测重点实验室, 湖北, 武汉, 430062
摘 要：
Objective: A novel method was developed for the determination of aflatoxin M_1 in milk by HPLC with on-line solid phase extraction (SPE). Methods: An on-line SPE column was prepared through packing SB-C_(18) filler in stainless column. The loading and washing solution were water, transferring solution was acetonitrile-water (20:80, V/V), and elution solution was acetonitrile-water (15:85, V/V). Results: Under optimized conditions, a linear regression equation was fitted as follows: Y = 2.52746X-0.067 with a correlation coefficient of 0.99906. The detection of limit (LOD) was 0.04 μg/kg, and the limit of quantification (LOQ) was 0.10 μg/kg. Average recovery rates for samples spiked at various levels were 98%-105.7% with RSD was 0.79%-2.56% (n = 6). Conclusion: A simple, accurate and credible method has been successfully developed for rapid determination of aflatoxin M_1 in milk.
