作 者：
陈小媚;丁小霞;喻理;王秀嫔;马飞;姜俊;胡小风;李培武
关键词：
在线固相萃取富集;高效液相色谱法;花生;白藜芦醇
摘 要：
建立了简便、快速测定花生中白藜芦醇的在线固相萃取富集-高效液相色谱法。固相萃取富集柱填料为dsDNA,在线固相萃取富集过程的上样溶剂为纯水,淋洗溶剂为纯水,转移溶剂为乙腈-水(80∶20,V/V),分析测定溶剂为乙腈-水(25∶75,V/V)。本方法对白藜芦醇检测的线性方程为Y=1 843.805X+39.179 1,相关系数为0.999 78;检出限(LOD)为0.004mg/kg;定量限(LOQ)为0.01mg/kg;基质4个水平添加回收率(n=6)在92.48%~107.98%;6次加标回收相对标准偏差(RSD)在0.19%~6.05%。
译 名：
Determination of resveratrol in peanut by high performance liquid chromatography spectrometry using on-line solid phase extraction
作 者：
CHEN Xiao-mei;DING Xiao-xia;YU Li;WANG Xiu-pin;MA Fei;JIANG Jun;HU Xiao-feng;LI Pei-wu;Oil Crops Research Institute,Chinese Academy of Agricultural Sciences;Key Laboratory of Detection for Mycotoxins,Ministry of Agriculture;Quality Inspection and Test Center for Oil seeds and Products,Ministry of Agriculture;Laboratory of Risk Assessment for Oilseeds Products,Ministry of Agriculture;Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture;
关键词：
On-line solid phase extraction;;High performance liquid chromatography(HPLC);;Peanut;;Resveratrol
摘 要：
A novel method was developed to determine resveratrol in peanut by HPLC with on-line solid phase extraction.The on-line soild phase extraction column was prepared by packing dsDNA column filler in stainless column.The loading and washing solution of the extraction column were both water,the transferring solution was acetonitrile-water(80∶ 20,V/V),and the determining solution was acetonitrile-water(25∶ 75,V/V).Under the above condition,the linear regress equations was Y = 1 843.805X + 39.179 1,with correlation coefficients of 0.999 78,detection limits of 0.004mg/kg,and quantification limits of 0.01mg/kg.The average recoveries of 4 levels spiking were 92.48% to 107.98%,the relative standard deviation(RSD) was 0.19% to 6.05%(n = 6).
