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当前位置: 首页 > 文章 > 鄂梨2号叶片高效再生体系的建立 湖北农业科学 2017 (23) 4613-4615
Position: Home > Articles > Establishment of High Frequency Regeneration System from Leaf of Eli 2 Hao Hubei Agricultural Sciences 2017 (23) 4613-4615

鄂梨2号叶片高效再生体系的建立

作  者：
涂俊凡;秦仲麒;李先明;伍涛;杨夫臣;朱红艳;刘政;杨立
关键词：
砂梨;鄂梨2号;组培苗叶片;再生
摘  要：
以早熟砂梨品种鄂梨2号组培苗叶片为外植体,建立叶片再生体系。结果表明,鄂梨2号叶片再生不定芽最佳培养基为NN69+1.0 mg/L TDZ+0.1 mg/L IBA,愈伤组织再生率达到100%,不定芽再生率为36.67%;不定芽增殖最佳培养基为MS+1.0 mg/L 6-BA+0.2 mg/L IBA,增殖系数为2.19;不定芽在1 000mg/L ABT中浸蘸5 s后接种在添加500 mg/L活性炭的1/2MS培养基上,生根率最高,为66.67%;试管苗移栽成活率为84.44%。
译  名：
Establishment of High Frequency Regeneration System from Leaf of Eli 2 Hao
作  者：
TU Jun-fan;QIN Zhong-qi;LI Xian-ming;WU Tao;YANG Fu-chen;ZHU Hong-yan;LIU Zheng;YANG Li;Institute of Fruit and Tea, Hubei Academy of Agricultural Sciences/Fruit and Tea Branch ,Hubei Innovation Center of Agricultural Science and Technology;
单  位：
Institute of Fruit and Tea, Hubei Academy of Agricultural Sciences/Fruit and Tea Branch ,Hubei Innovation Center of Agricultural Science and Technology
关键词：
sand pear;;Eli 2 Hao;;leaf;;regeneration
摘  要：
Leaves excised from Eli 2 Hao were used as explants to establish regeneration system. The results indicated that the maximum adventitious buds regeneration frequency achieved 36.67% on the mediu of NN69+1.0 mg/L TDZ+0.1 mg/L IBA.The most suitable medium for shoot multiplication was MS+1.0 mg/L 6-BA+0.2 mg/L IBA and the average multiplication coefficient was 2.19. The buds were dipped in 1 000 mg/L ABT for 5 s and inoculated on 1/2 MS medium with 500 mg/L activated carbon. The highest rooting rate was 66.67%, and the survival rate of plantlets was 84.44%.

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