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当前位置: 首页 > 文章 > 柔嫩艾美耳球虫serpin基因克隆与生物信息学分析 中国兽医学报 2013,33 (2) 212-216
Position: Home > Articles > Cloning and bioinformatics analysis of Eimeria tenella serpin gene Chinese Journal of Veterinary Science 2013,33 (2) 212-216

柔嫩艾美耳球虫serpin基因克隆与生物信息学分析

作  者：
李文超;顾有方;宫鹏涛;李建华;杨举;邱发贵;张西臣
单  位：
安徽科技学院动物科学学院;吉林大学畜牧兽医学院
关键词：
柔嫩艾美球虫;serpin基因;生物信息学分析
摘  要：
根据GenBank发表的Eimeria tenella serpin(Etserpin)基因设计1对引物,从孢子化卵囊总RNA中用RT-PCR方法扩增Etserpin基因,克隆后测序,应用生物信息学分析预测其核苷酸及其编码蛋白的结构与功能。结果表明,Etserpin开放阅读框为1 248bp,编码一分泌蛋白,N端具有1个28aa的信号肽,有1个跨膜区域,有2个糖基化位点和21个磷酸化位点,88,89,98～101,208～212,283～287,302～304区段是其可能的B细胞表位;同源性比对与进化树的分析表明其为抑制性serpin蛋白,结构保守,与E.acervulina、T.gondii和N.caninum亲缘关系较近。二级结构以!螺旋和随机卷曲为主,具有类似于serpin家族蛋白的三级结构。
译  名：
Cloning and bioinformatics analysis of Eimeria tenella serpin gene
作  者：
LI Wen-chao1,2,GU You-fang1,GONG Peng-tao2,LI Jian-hua2,YANG Jü2,QIU Fa-gui2,ZHANG Xi-chen2(1.College of Animal Science,Anhui science and Technology University,Fengyang,Anhui 233100,China;2.College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China)
关键词：
Eimeria tenella;serpin gene;bioinformatics analysis
摘  要：
Based on the published ORF of E.tenella serpin gene,a pair of primers were designed.The gene was amplified by RT-PCR from E.tenella total RNA,then cloned and sequenced.Subsequently,the structure and functions of this gene was analyzed by bioinformatics method.The results showed Etserpin had an open reading frame(ORF) of 1 248 bp encoded a secreted protein.the 1st to 18th amino acid residues of the N-teriminal represented signal peptide sequences,while there were one transmembrane peptides,two N-glycosylation sites and 21 phosphorylation sites.The B-cell epitopes were probably at its fragment No88-89,98-101,208-212,283-287,302-304.Homology analysis indicated that Etserpin was a structurally conserved,inhibitory serpin protein which had a close identity with serpin of E.acervulina,T.gondii and N.caninum.The Etserpin protein had the secondary structure mainly composed of a helix,random coil and similar tertiary structure of protein in the serpin superfamily.

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