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当前位置: 首页 > 文章 > 微波提取-高效液相色谱法测定食用菌中百草枯 食品科学 2013,34 (6) 188-191
Position: Home > Articles > Determination of Paraquat in Edible Fungi by Microwave Extraction-High Performance Liquid Chromatography FOOD SCIENCE 2013,34 (6) 188-191

微波提取-高效液相色谱法测定食用菌中百草枯

作  者：
唐俊;漆仲华;史陶中;吴祥为;操海群;花日茂;汤锋;岳永德
单  位：
安徽农业大学资源与环境学院;国际竹藤网络中心
关键词：
百草枯;食用菌;微波消解;固相萃取;高效液相色谱
摘  要：
建立百草枯在食用菌中的高效液相色谱残留分析方法。样品用10mL浓度2mol/L的盐酸溶液120℃微波消解提取30min,提取液经强阳离子交换(SCX)固相萃取小柱净化,氯化铵饱和溶液洗脱并定容。采用AtlantisHILIC Silica色谱柱,乙腈-辛烷基磺酸钠缓冲液为流动相,紫外检测波长259nm。该方法百草枯定量限为1.0μg/kg(RSN=10)。百草枯的添加回收率为82.33%～96.99%,相对标准偏差为1.23%～3.90%,该方法适合于食用菌中百草枯残留快速分析。
译  名：
Determination of Paraquat in Edible Fungi by Microwave Extraction-High Performance Liquid Chromatography
作  者：
TANG Jun1,2,QI Zhong-hua1,SHI Tao-zhong1,2,WU Xiang-wei1,2,CAO Hai-qun1,2,HUA Ri-mao1,2,TANG feng3,YUE Yong-de3,*(1.School of Resource and Enviroment,Anhui Agricultural University,Hefei 230036,China;2.Key Laboratory of Agri-food Safety of Anhui Province,Hefei 230036,China;3.International Centre for Bamboo and Rattan,Beijing 100102,China)
关键词：
paraquat;edible fungi;microwave digestion;solid-phase extraction(SPE);HPLC
摘  要：
An HPLC method was established for the determination of paraquat residues in edible fungi.Samples were extracted with 10 mL of hydrochloric acid solution by microwave digestion extraction at 120 ℃ for 30 min,cleanedup by strong cation exchange(SCX) solid-phase extraction(SPE) column and eluted by saturated ammonium chloride.HPLC separation was carried out by reverse phase chromatography on Atlantis HILIC silica column with a mobile phase composed of acetonitrile and actyl sulfonate at the ratio of 40:60.Paraquat was detected by ultraviolet detector at 259 nm.The limit of quantification(LOQ) based on 10 times signal-to-noise was 1.0 μg/kg.The recovery rate was in the range of 82.33%–96.99% with the relative standard deviations(RSD) of 1.23%–3.90%.This established method is satisfactory for the determination of paraquat residues in edible fungi.

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