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Position: Home > Articles > Optimization of Fermentation Conditions for Exopolysaccharide Production by Bacillus amyloliquefaciens LPL061 FOOD SCIENCE 2013,34 (7) 185-189

一株解淀粉芽孢杆菌产糖条件的优化

作  者:
李彦岩;张彩;范熠;刘丽莎;李平兰;韩玉竹
单  位:
中国农业大学食品科学与营养工程学院
关键词:
解淀粉芽孢杆菌;胞外多糖;条件优化;响应面分析法
摘  要:
为提高一株解淀粉芽孢杆菌LPL061胞外多糖(EPS)产量,采用单因素试验和响应面法,对该菌株的最适培养基成分和发酵条件进行优化.优化后培养基配方为:蔗糖22g/L、酵母膏18.4g/L、pH 7.0;发酵条件为:温度28℃、转速220r/min、接种量3%、发酵时间24h.优化后胞外多糖产量达4.46g/L,比优化前提高了1.51倍.
译  名:
Optimization of Fermentation Conditions for Exopolysaccharide Production by Bacillus amyloliquefaciens LPL061
单  位:
College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China
关键词:
Bacillus amyloliquefaciens%exopolysaccharide%fermentation condition optimization%response surface analysis
摘  要:
In order to improve expolysaccharide(EPS) production by Bacillus amyloliquefaciens LPL061,the medium and fermentation conditions were optimized by one-factor-at-a-time design and response surface analysis.The optimal culture medium consisted of 22 g/L sucrose and 18.4 g/L yeast extract at pH 7.0.The optimal fermentation conditions were 28 ℃,220 r/min shaking speed,3% inoculum size and 24 h.Under these conditions,the EPS yield was 4.46 g/L,1.51-fold higher than before the optimization.

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