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当前位置: 首页 > 文章 > 解淀粉芽孢杆菌H15产抗菌肽的发酵条件优化和提取方法比较研究 食品科学 2015,36 (15) 135-141
Position: Home > Articles > Optimization of Fermentation Conditions for Production of Antifungal Peptides by Bacillus amyloliquefaciens H15 and Comparison of Extraction Methods for Antifungal Peptides FOOD SCIENCE 2015,36 (15) 135-141

解淀粉芽孢杆菌H15产抗菌肽的发酵条件优化和提取方法比较研究

作  者：
韩玉竹;邓钊;张宝;游成真;丛苑;李平兰
单  位：
中国农业大学食品科学与营养工程学院;西南大学荣昌校区动物科学系
关键词：
抗菌肽;解淀粉芽孢杆菌;发酵条件;提取方法
摘  要：
以尖孢镰刀菌为指示菌,无菌滤液抑菌圈直径为考察指标,对解淀粉芽孢杆菌H15所产抗菌肽的发酵和提取条件进行优化。获得优化后的最佳培养基为L-1培养基,最佳培养条件为:接种量3%、初始pH 6.68、培养温度30.79℃、培养时间48.61 h、转速160 r/min。在此优化条件下,发酵液抑菌圈直径可高达25.21 mm,比优化前(16.40 mm)提高了53.48%。以4株玉米中携带的优势霉菌(草酸青霉、串珠镰刀菌、尖孢镰刀菌、黑曲霉)为指示菌,比较分析不同质量浓度硫酸铵沉淀和盐酸沉淀不同有机溶剂抽提对解淀粉芽孢杆菌H15抗菌肽提取的影响,结果均表明盐酸沉淀丙酮抽提法获得的抗菌肽活性最高。
译  名：
Optimization of Fermentation Conditions for Production of Antifungal Peptides by Bacillus amyloliquefaciens H15 and Comparison of Extraction Methods for Antifungal Peptides
作  者：
HAN Yuzhu;DENG Zhao;ZHANG Bao;YOU Chengzhen;CONG Yuan;LI Pinglan;Department of Animal Science, Rongchang Campus, Southwest University;College of Food Science and Nutritional Engineering, China Agricultural University;
关键词：
antifungal peptides;;Bacillus amyloliquefaciens;;fermentation condition;;extraction method
摘  要：
Fermentation and extraction conditions for antifungal peptide production by Bacillus amyloliquefaciens H15 were optimized for larger diameters of inhibitory zones of Fusarium oxysporum ACCC 37438 when exposed to cell-free fermentation broths. Results showed that using L-1 medium, the optimal culture conditions were 3%, 6.68, 160 r/min, 48.61 h and 30.79 ℃ for inoculum amount, initial p H, rotational speed, culture time and temperature, respectively, leading to an inhibitory zone diameter of 25.21 mm, which was 53.48% higher than that(16.40 mm) before optimization. In addition, extraction efficiencies of ammonia sulfate precipitation and hydrochloric acid precipitation followed by organic solvent extraction for the extraction of antifungal peptides from the fermentation broth were compared by using four dominant molds isolated from corn as the indicator strains. All the obtained results showed that hydrochloric acid precipitation and then acetone extraction yielded the highest antifungal activity.

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