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Position: Home > Articles > Establishment of a multiplex PCR assay for detection of common pathogens in food China Animal Health Inspection 2013 (10) 51-56

食品中常见病原菌多重PCR检测方法的建立

作  者:
王海源;赵志伟;莫国东;徐家芳;葛子汉;韦平
单  位:
广西大学养禽与禽病研究所
关键词:
多重PCR;沙门氏菌;空肠弯曲杆菌;单核细胞增生李斯特菌;大肠杆菌O157:H7;食品检验
摘  要:
根据基因库中沙门氏菌、空肠弯曲杆菌、单核细胞增生李斯特菌和大肠杆菌O157:H7的invA、MapA、hlyA和O gene cluster基因分别设计了4对引物,通过对反应条件的优化,建立了同时检测4种病原菌的多重PCR方法。结果表明,该多重PCR方法可扩增出四条特异性条带,并且任意两条产物片段长度相差大于20%。多重PCR反应体系检测四种病原菌混合模板最低含量为100 CFU。该多重PCR检测方法具有快速、准确和特异性强的优点,可用于快速检测食品中的病原菌。
译  名:
Establishment of a multiplex PCR assay for detection of common pathogens in food
作  者:
Wang Haiyuan;Zhao Zhiwei;Mo Guodong;Xu Jiafang;Ge Zihan;Wei Ping;Institute of Poultry Science & Health,Guangxi University;
关键词:
Multiplex-PCR assay;;Salmonella;;Campylobacter jejuni;;Listeria monocytohenes;;Escherichia coli O157:H7;;food inspection
摘  要:
Four pairs of primers were designed according to the sequences of invA gene of Salmonella,MapA gene of Campylobacter jejuni,hlyA gene of Listeria monocytohenes and O gene cluster of Escherichia coli O157:H7. After optimization of conditions,a multiplex-PCR assay was established for rapid detection of the 4 common pathogens in food.Results showed that the multiplex PCR assay could amplify four specific bands and more than 20% difference in length existed between any two of them,and the sensitivity of the PCR system was at least 100 CFU mixed templates of the 4pathogens. The multiplex-PCR assay was rapid,accurate and specific in operation and could be used in food inspection.

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