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Position: Home > Articles > Comparison of the Content of Adenosine Contained in RHIZOMA ET RADIX BAPHICACANTHIS CUSIAE from Different Production Places in Guangdong Province Journal of Anhui Agricultural Sciences 2012,40 (26) 12852-12854

广东不同产地南板蓝根药材中腺苷含量的测定

作  者:
李世杰;陈奕龙;张丹雁
单  位:
广州中医药大学中药学院
关键词:
南板蓝根;腺苷;HPLC
摘  要:
[目的]测定广东不同产地南板蓝根药材中有效成分腺苷的含量。[方法]建立南板蓝根药材中腺苷的HPLC含量测定方法;色谱条件为:ZORBAX Extend-C18色谱柱(4.6 mm×250 mm,5μm);流动相为无水甲醇-水(7∶93,V/V);检测波长为260 nm;流速为1.0ml/min;柱温为30℃。[结果]在4.65~232.6 ng/ml范围内,腺苷浓度与峰面积有良好的线性关系,加样回收率为100.3%,RSD为2.2%(n=6);广东从化市样品中的腺苷含量最高,2种野生品种含量最低。[结论]该方法操作简便、准确快速、重现性好,可有效控制南板蓝根药材的质量。
译  名:
Comparison of the Content of Adenosine Contained in RHIZOMA ET RADIX BAPHICACANTHIS CUSIAE from Different Production Places in Guangdong Province
作  者:
LI Shi-jie et al(College of Chinese Materia Medica,Guangzhou University of Chinese Medicine,Guangzhou,Guangdong 510405)
关键词:
RHIZOMA ET RADIX BAPHICACANTHIS CUSIAE;Adenosine;HPLC
摘  要:
[Objective] To make the use of high performance liquid chromatographic method(HPLC) to determine the content of active ingredient adenosine in RHIZOMA ET RADIX BAPHICACANTHIS CUSIAE from different production places in Guangdong Province.[Method] A HPLC method was established for determining the content of adenosine.To be specific,HPLC analysis was performed on a chromatographic column ZORBAXExtend-C18(4.6 mm×250 mm,5 μm) with methanol-water(V/V,7∶93)as the mobile phase,detection wavelength at 260 nm,flow rate at 1.0 ml/min and column temperature at 30 ℃.[Result] Adenosine had good linear relationship between concentration and peak area from 4.65 to 232.6 ng,the recovery rate was 100.3%,RSD=2.2%(n=6);the sample from Conghua in Guangdong Province had the highest content,while the two wild species had the lowest of that.[Conclusion] The method is simple,accurate,rapid and reproducible.It can complete the existing quality standard of RHIZOMA ET RADIX BAPHICACANTHIS CUSIAE compound feed additives,and make the control of quality efficiently.

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