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当前位置: 首页 > 文章 > 绵羊KAP1.1基因克隆与单核苷酸多态性分析 西南农业学报 2010,23 (5) 1720-1724
Position: Home > Articles > Cloning and Polymorphism of KAP1.1 Gene in Sheep Southwest China Journal of Agricultural Sciences 2010,23 (5) 1720-1724

绵羊KAP1.1基因克隆与单核苷酸多态性分析

作  者：
冯静;杨华;宋天增;杨剑波;石国庆;徐从祥;黄锡霞
单  位：
石河子大学生命科学学院;新疆农垦科学院畜牧兽医研究所;新疆农业大学动物科技学院
关键词：
中国美利奴羊(新疆军垦型);KAP1.1;SNP;基因克隆
摘  要：
本研究以768只中国美利奴羊(新疆军垦型)为实验材料,采用测序和PCR技术分析KAP1.1(B2A)基因单核苷酸多态性。测序表明中国美利奴羊(新疆军垦型)KAP1.1基因存在30 bp的插入/缺失,12%PAGE电泳证实KAP1.1基因存在6种基因型:(341 bp)AA型、(311 bp)BB型、(281 bp)CC型、(341 bp/311 bp)AB型、(341 bp/281 bp)AC型和(311 bp/281 bp)BC型,基因型频率依次为0.0208、0.5534、0.0260、0.1589、0.0456和0.1953。检测到A、B和C3个等位基因,基因频率依次为0.1230、0.7305和0.1465。研究结果将为开展优质细毛羊毛性状功能基因的研究提供基础。
译  名：
Cloning and Polymorphism of KAP1.1 Gene in Sheep
作  者：
FENG Jing1,2,YANG Hua2,SONG Tian-zeng2,4,YANG Jian-bo2,SHI Guo-qing2,XU Cong-xiang3,HUANG Xi-xia1(1.College of Animal Science and Technology,Xinjiang Agricultural University,Xinjiang Urumqi 830052,China;2.Institute of Animal Husbandry and Veterinary,Xinjiang Academy of Agriculture and Reclamation Science,Xinjiang Shihezi 832000,China;3.College of Life Science,Shihezi University,Xinjiang Shihezi 832000;4.Institute of Animal Husbandry Science,Sichuan Kangding 626000,China)
关键词：
Chines Merino(XinJiang JunKen Type);KAP1.1;SNP;Gene cloning
摘  要：
Sequencing and SNP analysis was used to identify sequence variation in KAP1.1(B2A)gene.12% PAGE electrophoresis was used to identify length polymorphism in the KAP1.1 gene.The allelic variation was confirmed by DNA sequencing.The results showed three KAP1.1 alleles were identified which varied in length due to the insertion or deletion of 30 nucleotides.There were seven genotypes of KAP1.1 gene,which were(341 bp)AA genotype,(311 bp)BB genotype,(281 bp)CC genotype,(341 bp/311 bp)AB genotype,(341 bp/281 bp)AC genotype and(311 bp/281 bp)BC genotype,respectively.Genotypic frequency were by turns 0.0208,0.5534,0.0260,0.1589,0.0456 and 0.1953.A,B and C allele frequency were 0.1230,0.7305 and 0.1465.This study would provide basis for finding functional gene of wool trait.

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