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Position: Home > Articles > ACE Inhibitory Activity of Hydrolysates of Phycobiliproteins from Spirulina FOOD SCIENCE 2009,30 (13) 212-217

螺旋藻藻胆蛋白水解产物对ACE抑制活性的研究

作  者：
刘立闯;胡志和;贾静;刘彤
单  位：
天津市食品生物技术重点实验室;天津市食品生物技术重点实验室,天津商业大学生物技术与食品科学学院
关键词：
藻胆蛋白;胃蛋白酶;胰蛋白酶;ACE;抑制活性
摘  要：
采用胃蛋白酶和胰蛋白酶水解螺旋藻藻胆蛋白制备ACE抑制肽,通过体外实验测定其ACE抑制率,以ACE抑制率为指标确定两种蛋白酶的水解条件。结果表明:胃蛋白酶水解条件为:水解温度37℃,酶与底物比1:50,pH3.0,底物质量分数5%,水解产物的ACE抑制率为82.16%,其IC50值为0.104mg/ml;胰蛋白酶水解条件为:水解温度42℃,酶与底物1:50,pH8.0,底物质量分数6%,水解产物的ACE抑制率为93.54%,其IC50值为0.017mg/ml。此外,胃蛋白酶水解产物再用胰蛋白酶水解,其产物的IC50值为0.087mg/ml。
译  名：
ACE Inhibitory Activity of Hydrolysates of Phycobiliproteins from Spirulina
作  者：
LIU Li-chuang,HU Zhi-he,JIA Jing,LIU Tong (Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China)
关键词：
phycobiliprotein;pepsin;trypsin;ACE;inhibitory activity
摘  要：
ACE inhibitory peptides were prepared from Spirulina phycobiliproteins by pepsin or trypsin-catalyzed hydrolysis and the ACE inhibitory activities were determined by in vitro spectrophotometric assay so as to optimize the hydrolysis conditions by orthogonal factorial design. The results showed the conditions for pepsin-catalyzed hydrolysis were as follows: enzyme/substrate ratio 1:50, pH 3.0 and substrate concentration 5% (m/V), and hydrolysis temperature 37 ℃ for a hydrolysis duration of 120 min, and these conditions resulted in 82.16% of the ACE inhibition rate and the IC50 reached 0.104 mg/ml; the optimal conditions for trypsin-catalyzed hydrolysis were as follows: enzyme/substrate ratio 1:50, pH 8.0 and substrate concentration 6% (m/V), and hydrolysis temperature 42 ℃ for a hydrolysis duration of 160 min; and the IC50 of trypsin-catalyzed hydrolysates of Spirulina phycobiliproteins by pepsin (both under the optimized conditions) reached 0.087 mg/ml.

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