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Position: Home > Articles > Study on Physiological and Biochemical Defense Responses of Grape Against Infection of Plasmopara viticola Shandong Agricultural Sciences 2018 (1) 122-126

葡萄抵御霜霉病菌侵染的生理生化防卫反应研究

作  者:
李宝燕;王英姿;姜法祥;石洁;刘学卿
单  位:
烟台市农业科学研究院
关键词:
葡萄;霜霉病菌;不同品种;生理生化反应
摘  要:
为给葡萄抗霜霉病育种研究提供理论参考,通过田间调查结合室内接种的方法测定不同品种葡萄对霜霉病的抗性;测定接种葡萄霜霉病菌后,不同抗性品种叶片的SOD、POD、CAT活性及SA、H_2O_2、JA含量的变化;观察喷施SA、H_2O_2、JA对葡萄霜霉病抗性的影响,明确萄萄抵御霜霉病菌侵染的生理生化防卫反应。结果表明,与感病品种相比,抗性品种的POD、CAT活性在接种病菌后迅速增强;不同品种的SA、H2O2含量在接种病原菌后与对照相比都有明显升高,抗病品种升高出现早、生成量更高;不同品种叶片JA含量较对照虽有增加,但品种间无差异显著性;水杨酸、茉莉酸、过氧化氢有助于增强葡萄对霜霉病的抗性,水杨酸增强抗性较为明显,抗性增强44.87%。
译  名:
Study on Physiological and Biochemical Defense Responses of Grape Against Infection of Plasmopara viticola
作  者:
Li Baoyan;Wang Yingzi;Jiang Faxiang;Shi Jie;Liu Xueqing;Yantai Academy of Agricultural Sciences;
关键词:
Grage;;Plasmopara viticola;;Different varieties;;Physiological and biochemical response
摘  要:
In order to provide theoretical references for grape resistance breeding against Plasmopara viticola,field investigation and indoor inoculation were used to detect the grape resistance to Plasmopara viticola.The changes of SOD,POD and CAT activities and SA,H_2O_2 and JA contents in leaves of different resistant varieties were determined after inoculation with Plasmopara viticola. The effects on grape resistance to Plasmopara viticola were observed after spraying SA,H_2O_2 and JA to clarify the physiological and biochemical defense responses of grape against the infection of Plasmopara viticola. The results showed that,compared with the susceptible varieties,the activities of POD and CAT were rapidly increased after inoculation. The contents of SA and H_2O_2 in different varieties increased obviously after inoculation compared with the control,and the resistant varieties showed earlier and higher formation amounts. JA content increased in different varieties,but there were no significant differences between cultivars. SA,JA and H_2O_2 were helpful to enhance the resistance of grape to Plasmopara viticola. SA enhanced the resistance obviously and the resistance was enhanced by 44. 87%.

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