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当前位置: 首页 > 文章 > 番石榴实蝇和瓜实蝇的线粒体L-rRNA基因测序及双翅目昆虫L-rRNA基因的结构特征与系统发育关系分析 昆虫学报 2012,55 (8) 39-50
Position: Home > Articles > Sequencing of mitochondrial L-rRNAgenes of Bactrocera cucurbitae and B. correcta (Diptera:Tephritidae) and the analysis of structural characteristics and phylogenetic relationship of L-rRNA genes in dipterans Acta Entomologica Sinica 2012,55 (8) 39-50

番石榴实蝇和瓜实蝇的线粒体L-rRNA基因测序及双翅目昆虫L-rRNA基因的结构特征与系统发育关系分析

作  者：
吴培福;熊忠平;徐磊;黄阳洋;潘涌智
单  位：
西南林业大学云南省森林灾害预警与控制重点实验室
关键词：
双翅目;番石榴实蝇;瓜实蝇;L-rRNA基因;测序;二级结构;结构特征;系统发育
摘  要：
RNA二级结构比一级结构包含更多的信息,在物种系统发育分析中更能反应真实情况。因而,本研究对番石榴实蝇Bactrocera correcta和瓜实蝇B. cucurbitae的L-rRNA全基因进行了测序,构建了双翅目L-rRNA基因二级结构的模式图,并分析了其结构特征,而后基于H45～H47茎环结构参数和结构序列及L-rRNA结构序列对双翅目13科的系统发育关系进行了初步分析。结果表明:双翅目昆虫具有保守的L-rRNA二级结构;不同结构区碱基组成和分布不均匀,Ⅳ区和Ⅴ区的完全保守碱基含量最高,GC含量最高,绝大部分科Ⅵ区的AT斜率均小于0;科内特有碱基和科间少数保守碱基大部分为G或C。瘿蚊科与双翅目其他科具有较远的系统发育关系,而丽蝇科、寄蝇科和食蚜蝇科有相近的系统发育关系;虻科与网翅虻科同在一个小分支;蠓科与蚊科同在一个大的系统发育分支。利用单方面参数不易得到理想的进化结果,准确系统发育分析需要结合多方面的参数。
译  名：
Sequencing of mitochondrial L-rRNAgenes of Bactrocera cucurbitae and B. correcta (Diptera:Tephritidae) and the analysis of structural characteristics and phylogenetic relationship of L-rRNA genes in dipterans
作  者：
WU Pei-Fu ,XIONG Zhong-Ping ,XU Lei,HUANG Yang-Yang,PAN Yong-Zhi ( Key Laboratory of Forest Disaster Warning and Control in Yunnan Province,Southwest Forestry University,Kunming 650224,China)
关键词：
Diptera;Bactrocera correcta;Bactrocera cucurbitae;L-rRNA gene;sequencing;secondary structure;structural characteristics;phylogenesis
摘  要：
The RNA secondary structure contains more information than its primary structure,and can indicate the phylogenetic relationship more really. Therefore,the complete sequences of mitochondrial L-rRNA genes of Bactrocera cucurbitae and B. correcta were sequenced in this study,and the structural model of L-rRNA genes in Diptera was constructed. The characteristics of L-rRNA secondary structure was analyzed,and preliminary phylogenetic features were inferred based on stem-loop parameters and structural sequences of H45 - H47,and structural sequences of L-rRNA genes among 13 families in Diptera. The results showed that the dipterans share the conserved secondary structure of L-rRNA gene; the nucleotide composition and distribution vary within different structure regions of L-rRNA gene; the highest content of completely conserved nucleotides was found in region Ⅳ and Ⅴ; the region Ⅳ and Ⅴ also contains the highest GC content; the AT-skew is negative in region Ⅵ of L-rRNA gene of the majority of dipteran families; the unique bases within individual family and individually conserved nucleotides between families present higher content of G or C. The family Cecidomyiidae exhibits much farther distance with other dipteran families,while Calliphoridae,Tachinidae and Syrphidae share much closer phylogenetic relationship; Tabanidae and Nemestrinidae belong to a small branch; Ceratopogonidae and Culicidae share a large phylogenetic branch. The satisfied phylogenetic result would not be obtained easily using single phylogenetic parameter,and it is thus necessary to infer accurate phylogenesis through combined parameters of different features.

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