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Position: Home > Articles > A simple and efficient DNA purification method Journal of Beijing University of Agriculture 2015,30 (4) 29-32

一种简便有效的核酸纯化方法

作  者:
杜瑞晓;张璐璐;陈楠;沈利斌;张天宇;吕鹤书;马兰青;杨明峰
单  位:
农业部都市农业(北方)重点实验室/北京农学院生物科学与工程学院
关键词:
核酸;PCR产物;纯化效率;纯度
摘  要:
核酸纯化是分子生物学实验中最常用的技术之一,现有的常规核酸纯化方法比较费时费力。结合经典的醇沉淀法与质粒提取试剂盒二者的优点,探索出改良柱纯化法,即在核酸溶液中加入适量的醇和醋酸钠,用硅胶柱快速回收核酸。试验证明新方法与现有方法的纯化效率相近,节约试验成本,且操作简单快速。纯化后的DNA能够满足酶切分析、细菌转化等试验的需要。
译  名:
A simple and efficient DNA purification method
作  者:
DU Ruixiao;ZHANG Lulu;CHEN Nan;SHEN Libin;ZHANG Tianyu;LüHeshu;MA Lanqing;YANG Mingfeng;Key Laboratory of Urban Agriculture(North)Ministry of Agriculture/Beijing University of Agriculture;
关键词:
DNA;;PCR product;;purification efficiency;;purity
摘  要:
DNA purification is one of the most commonly used techniques in molecular biology experiments.Current protocols of DNA purification are complex and time-consuming.In this paper,we exploited the advantages of alcohol precipitation of DNA method and Plasmid Extraction Kit method,by adding alcohol and sodium acetate into nucleic acid solution before recovering DNA using DNA binding column.We compare the new protocol with current ones,and the result indicates that the new scheme saves both time and labour with a high recovering rate.The purified DNA can be used in molecular experiments,such as enzyme digestion,bacterial transformation,etc.

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