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当前位置: 首页 > 文章 > 产朊假丝酵母核酸高产株的选育及其发酵性能研究 食品科学 2017,38 (10) 149-154
Position: Home > Articles > Breeding of a Candida utilis Mutant for Efficient Nucleic Acid Production and its Fermentation Performance FOOD SCIENCE 2017,38 (10) 149-154

产朊假丝酵母核酸高产株的选育及其发酵性能研究

作  者：
蒋雪薇;岳希洁;陈代文;周志明;罗晓明;叶菁;李晓文
单  位：
连南瑶族自治县奇乡生物科技有限公司;长沙理工大学化学与生物工程学院
关键词：
产朊假丝酵母;复合诱变;核酸;流加培养
摘  要：
为了获得一株发酵性能优良的高核酸酵母,以产朊假丝酵母(Candida utilis)CL1501为出发菌株,采用紫外-亚硝基胍复合诱变,筛选获得一株高核酸含量的突变株CL15013,经测定其核酸含量占菌体干质量的16.8%,高于出发菌47.8%。对比研究其流加及分批培养工艺,流加培养细胞收获量为16.9 g/L,比分批培养提高94.3%;正交试验设计优化流加培养条件后,CL15013的收获量达21.3 g/L,比分批培养提高144.8%,具有良好的生产应用潜力。
译  名：
Breeding of a Candida utilis Mutant for Efficient Nucleic Acid Production and its Fermentation Performance
作  者：
JIANG Xuewei;YUE Xijie;CHEN Daiwen;ZHOU Zhiming;LUO Xiaoming;YE Jing;LI Xiaowen;College of Chemical and Biological Engineering,Changsha University of Science & Technology;Liannan Yao Autonomous County Qixiang Bio-Sci Co.Ltd.;
关键词：
Candida utilis;;combined mutation;;nucleic acid;;fed-batch culture
摘  要：
Candida utilis CL1501 was mutagenized with a combination of UV irradiation and nitrosoguanidine treatment. An excellent mutant with high ability to produce nucleic acid, named as CL15013, was obtained. Its nucleic acid content was 16.8% on a dry cell weight basis, which was 47.8% higher than that of CL1501. The dry biomass yield of the mutantwas 16.9 g/L in fed-batch culture, which was 94.3% higher than that from batch culture. Under optimized fed-batch culture conditions obtained by orthogonal array design, the biomass yield was up to 21.3 g/L, which was 144.8% higher than that from batch cultivation. These results indicated that mutant CL15013 has a great application potential in industrial production.

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