中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 新浦东鸡DNA指纹的随机扩增多态分析 中国家禽 2003,7 (1)
Position: Home > Articles > RAPD Analysis of DNA Fingerprinting on NewPudong Chicken China Poultry 2003,7 (1)

新浦东鸡DNA指纹的随机扩增多态分析

作  者：
张丽娟;杨长锁;严华祥;钱春梅;耿社民;王秋丽
单  位：
上海国家家禽工程技术研究中心;华南农业大学生物技术学院;西北农林科技大学动物科技学院
关键词：
新浦东鸡;DNA指纹;随机引物;遗传多样性
摘  要：
利用随机扩增多态DNA指纹分析技术,由12个随机引物中筛选出3个具有多型的引物对28只新浦东鸡的基因组DNA指纹进行研究。结果表明:3个引物共扩增出27条DNA片段,多型条带24条,其多型频率为0.593;其H05引物扩增出DNA片段7条,其多型频率为0.259,G04引物扩增出DNA片段11条,其多型频率为0.408;V15引物扩增出DNA片段9条,其多型频率为0.333,3个引物扩增的DNA片段长度为489~1940bp。各位点的片段共享度分别是0.856,0.716和0.674;杂合度为0.549,0.695和0.727;平均百分差异为0.144,0.284和0.326;平均百分差异均值为0.251。
译  名：
RAPD Analysis of DNA Fingerprinting on NewPudong Chicken
作  者：
Zhang Lijuan,Yang Changsuo,Yan Huaxiang,Qian Chunmei,Geng Shemin,Wang Qiuli(National Poultry Technology Center,Shanghai 200331;College of Animal Science & Technology,Northwestern Sci-Tech University of Agriculture &Forestry,Yangling 712100;South China Agriculture University,Guangdong 510000 China)
关键词：
New Pudong chicken;DNA fingerprinting;random primer;genetic polymorphism
摘  要：
The 3 polymorphism primers selected from 12 random primers were used in the population genetic analysis of New Pudong chicken. The results showed that.-27 bands were amplified from the 3 polymorphism primers, 24 of which were polymorphous. 7,11 and 9 DNA fragments were amplified by H05 ,G04 and V15; Polymorphism frequencies was 0.259,0.408 and 0.333 .The length of DNA fragments were between 489-1940bp.The average polymorphism frequency band sharing rates were 0.856,0.716 and 0.674 respectively. The heterozygosities were 0.549,0.695 and 0.727,and the average percentage differences were 0.144,0.284 and 0.326 for H05.G04 and V15 primers. The mean of average percentage difference was 0.251.

相似文章

导出引用
计量
文章访问数: 11
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46