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当前位置: 首页 > 文章 > 环糊精功能化修饰纳米银负载姜黄素的制备及其抗肿瘤活性 食品科学 2017,38 (21) 38-42
Position: Home > Articles > Preparation and Anticancer Activity of Cyclodextrin-Functionalized Curcumin-Loaded Silver Nanoparticles FOOD SCIENCE 2017,38 (21) 38-42

环糊精功能化修饰纳米银负载姜黄素的制备及其抗肿瘤活性

作  者：
陈建平;余苗;秦小明;谌素华;钟赛意
单  位：
广东省天然产物绿色加工与产品安全重点实验室;江西省粮油质量监督检验中心;广东海洋大学食品科技学院
关键词：
姜黄素;β-环糊精;银纳米粒子;制备;抗肿瘤活性
摘  要：
采用纳米银材料制备负载姜黄素(curcumin,Cur)的β-环糊精(β-cyclodextrin,β-CD)功能化纳米银——Ag@β-CD@Cur,并进一步对其抗肿瘤活性进行测定。运用透射电子显微镜和纳米粒度仪鉴定Ag@β-CD@Cur的表面形貌、粒径大小和稳定性。运用倒置荧光显微镜和3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐法检测Ag@β-CD@Cur对人肝癌细胞HepG2形态和活性的影响,并进一步采用流式细胞术检测细胞凋亡比例。实验结果表明,所制备的Ag@β-CD@Cur是纯度很高的单质银,且大小均一、表面光滑,粒径约为2 nm。同时,Ag@β-CD@Cur具有较好的稳定性,能在水中稳定存在30 d。经Ag@β-CD@Cur处理HepG2细胞24 h后,HepG2细胞的形态出现明显的变圆、细胞核固缩等细胞凋亡特征,同时,细胞存活率从对照组的100%降低到24%,进一步的流式细胞术分析结果表明,细胞凋亡比例从对照组的2.5%增加到51.0%。实验结果表明,纳米化后的Cur具有较强的抗肿瘤活性效果,这为Cur在纳米制剂的应用提供了一定的技术手段和理论数据参考。
译  名：
Preparation and Anticancer Activity of Cyclodextrin-Functionalized Curcumin-Loaded Silver Nanoparticles
作  者：
CHEN Jianping;YU Miao;QIN Xiaoming;CHEN Suhua;ZHONG Saiyi;College of Food and Technology,Guangdong Ocean University;Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety;Grain and Edible Oil Products Quality's Supervision and Inspection Center in Jiangxi Province;
单  位：
College of Food and Technology,Guangdong Ocean University%Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety%Grain and Edible Oil Products Quality's Supervision and Inspection Center in Jiangxi Province
关键词：
curcumin;;β-cyclodextrin;;silver nanoparticles;;preparation;;anticancer activity
摘  要：
In this study,cyclodextrin-functionalized curcumin-loaded silver nanoparticles(Ag@β-CD@Cur) were prepared with silver nanomaterials and its anticancer activity was also studied.The surface morphology,particle size and stability of Ag@β-CD@Cur were detected by transmission electronic microscopy and Zetasizer Nano.Moreover,the effect of Ag@β-CD@Cur on morphological and viability changes of HepG2 cells were detected by phase-contrast microscopy and(3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2 H-tetrazolium bromide assay.Moreover,the population of apoptotic cells was detected by flow cytometric analysis.The experimental results showed that Ag@β-CD@Cur was obtained as highly pure,uniformly sized silver nanoparticles with a smooth surface.The average size of Ag@β-CD@Cur was 2 nm.Moreover,Ag@β-CD@Cur had good stability in water for 30 days.After treatment of HepG2 cells with Ag@β-CD@Cur for 24 h,apoptosis characteristics appeared such as cell swelling and cell nuclei pyknosis.At the same time,the cell viability reduced to 24% of the control group.Further flow cytometric analysis showed that the population of apoptotic cells increased from 2.5%(control) to 51.0%.The results demonstrated that after nanoparticlization,curcumin has stronger anticancer activity,which will provide a technical means and theoretical data for curcumin nano-formulation.

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