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Position: Home > Articles > Cloning and nucleotide sequence analysis of the p35 gene of Hyphantria cunea nucleopolyhedrovirus Acta Entomologica Sinica 2002,45 (6) 8-13

美国白蛾核型多角体病毒p35基因的克隆及序列分析

作  者:
曹广力;薛仁宇;朱越雄;魏育红;贡成良
单  位:
苏州大学生命科学学院
关键词:
美国白蛾核型多角体病毒;p35基因;核苷酸序列
摘  要:
对美国白蛾核型多角体病毒 (HycuNPV ,Hyphantriacuneanucleopolyhedrovirus)p35基因的序列分析表明 :HycuNPVp35编码序列 90 0bp ,编码 2 99氨基酸。同源性分析表明 :HycuNPVp35与BomoNPVT3、AucaNPV、SpliNPV、LeseNPV、HearNPV在核苷酸水平上为 99 9%、 95 7%、 93 6 %、 80 2 %和 87 2 % ,在氨基酸水平上为 99 7%、 90 3%、 77%、 6 4 9%和 73 2 % ,显示了杆状病毒p35基因在进化上的保守性。BomoNPVT3中位的H1 2 2 ,在HycuNPV中被R取代。推测HycuNPVp35蛋白的功能及抑制细胞凋亡的能力与BomoNPVT3p35蛋白的相似。
译  名:
Cloning and nucleotide sequence analysis of the p35 gene of Hyphantria cunea nucleopolyhedrovirus
作  者:
CAO Guang Li, XUE Ren Yu, ZHU Yue Xiong, WEI Yu Hong, GONG Cheng Liang * (Life Science College of Suzhou University, Suzhou 215006, China)
关键词:
Hyphantria cunea nucleopolyhedrovirus; p35 gene; nucleotide sequence
摘  要:
Sequence analysis of the p35 gene of Hyphantria cunea nucleopolyhedrovirus (HycuNPV) showed that the HycuNPV p35 gene was 900 bp and encoded 299 amino acids. Compared with BomoNPV T3, AucaNPV, SpliNPV, LeseNPV and HearNPV, nucleotide and amino acid identity were 99 9%, 95 7%, 93 6%, 80 2%, 87 2% and 99 7%, 90 3%, 77%, 64 9%, 73 2% respectively, and showed evolutionary conservation. H 122 of BomoNPV T3 p 35 was replaced by R in HycuNPV p 35 It is possible that the function and anti apoptotic action of HycuNPV p 35 is similar to that of BomoNPV T3 p 35.

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