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当前位置: 首页 > 文章 > 酶法辅助超声波法提取类球红细菌SOD工艺研究 沈阳农业大学学报 2014,1 (1) 47-51
Position: Home > Articles > Optimization of Ultrasonic Assisted with Zymolysis Extraction Technique of SOD from Rhodobacter sphaeroides Journal of Shenyang Agricultural University 2014,1 (1) 47-51

酶法辅助超声波法提取类球红细菌SOD工艺研究

作  者：
李祖明;霍笑靓;高丽萍;惠伯棣;杨卫东;王栋;白志辉
单  位：
北京联合大学应用文理学院;北京天维新探生物科技有限公司;中国科学院生态环境研究中心
关键词：
类球红细菌;超氧化物歧化酶(SOD);酶法辅助超声波法;正交试验
摘  要：
为了研究类球红细菌SOD的提取工艺,采用研磨法、超声波法、冻融辅助超声波法和酶法辅助超声波法提取类球红细菌超氧化物歧化酶,结果表明:酶法辅助超声波法是较优的提取方法。对类球红细菌SOD的提取工艺进行单因素和正交试验,得到较优的提取工艺参数为:超声波输出功率500W,超声波工作总时间15min,固液比1∶15,酶添加量300μL,酶解温度40℃,酶解pH值7,酶解时间60min。在此较优提取工艺参数下,类球红细菌SOD活性达到385.7 U·mL-1,较优化前提高40%。
译  名：
Optimization of Ultrasonic Assisted with Zymolysis Extraction Technique of SOD from Rhodobacter sphaeroides
作  者：
LI Zu-ming;HUO Xiao-liang;GAO Li-ping;HUI Bo-di;YANG Wei-dong;WANG dong;BAI Zhi-hui;College of Arts and Sciences, Beijing Union University;Beijing Tianweixintan Biotechnology Company Ltd.;The Research Center for Eco-Enviromental Sciences,Chinese Academy of Sciences;
关键词：
Rhodobacter sphaeroides;;superoxide dismutase;;ultrasonic assisted with zymolysis;;orthogonal test
摘  要：
Extraction technique of SOD from Rhodobacter sphaeroides was studied by ultrasonic assisted with zymolysis. By comparision with grinding, ultrasonic and ultrasonic assisted with freezing-thawing, ultrasonic assisted with zymolysis was the optimum extraction method for superoxide dismutase(SOD) from Rhodobacter sphaeroides 3757. Results of both single factor experiment and orthogonal test showed that the optimum ultrasonic assisted with zymolysis extraction conditions of SOD were as follows: ultrasonic power 500W, ultrasonic total working time 15min, material/liquid ratio 1 ∶15, amount of enzyme 300 μL, temperature of zymoIysis 40℃, pH of zymoIysis 7, time of zymoIysis 60 min. Under these optimum conditions, activities of SOD was 385.7 U·mL-1, and the extraction rate would be improved by 40%.

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