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当前位置: 首页 > 文章 > 侵染曼陀罗的中国番茄黄化曲叶病毒及其卫星DNAβ全基因组结构特征 植物病理学报 2008,38 (4) 364-369
Position: Home > Articles > Molecular characterization of Tomato yellow leaf curl China virus and its associated satellite DNAβ infecting Datura stramonium L. Acta Phytopathologica Sinica 2008,38 (4) 364-369

侵染曼陀罗的中国番茄黄化曲叶病毒及其卫星DNAβ全基因组结构特征

作  者：
丁铭;杨程;罗延青;岳宁;方琦;苏晓霞;张仲凯
单  位：
云南省大理州烟草公司;云南省农业科学院生物技术与种质资源研究所
关键词：
中国番茄黄化曲叶病毒;曼陀罗;菜豆金色黄花叶病毒属;卫星DNAβ
摘  要：
从云南大理曼陀罗上采集到病毒分离物YN72,症状表现为叶脉增厚、叶片褪绿、植株矮化。全序列测定表明,YN72DNA-A全长2 739个核苷酸。基因组比较发现,YN72 DNA-A与中国番茄黄化曲叶病毒分离物(TYLCCNV-[Y43])同源性最高(93.4%),与中国番茄黄化曲叶病毒烟草分离物(TYLCCNV-[Y5])的同源性次之(92.9%),而与亚洲地区的其它双生病毒的同源性均在90%以下,表明曼陀罗中的分离物YN72是TYLCCNV的1个分离物。利用W TG s卫星分子DNAβ的特异性引物beta01和beta02,在YN72中PCR扩增到DNAβ分子。序列分析表明,YN72β全长1 335个核苷酸,在其互补链上编码1个有功能的ORF(C1)。YN72β的全序列与TYLCCNV分离物卫星分子Beanβ和Y297β的同源性最高,分别为99.8%和99.3%;与其它所比较的DNAβ的同源性均低于80.6%。系统进化树研究表明,YN72卫星分子DNAβ与其辅助病毒是共同进化的。
译  名：
Molecular characterization of Tomato yellow leaf curl China virus and its associated satellite DNAβ infecting Datura stramonium L.
作  者：
DING Ming1,YANG Cheng2,LUO Yan-qing1,YUE Ning1,FANG Qi1,SU Xiao-xia1,ZHANG Zhong-kai1(1Biotechnology and Genetic Resources Institute,YAAS,Kunming 650223,China;2Dali Tobacco Company of Yunnan Province,Dali 671000,China)
关键词：
Tomato yellow leaf curl China virus;Datura stramonium L.;Begomovirus;satellite DNAβ
摘  要：
Virus isolate YN72 was obtained from Jimsonweed(Datura stramonium L.) showing vein thic-kening,leaf curling and stunt symptoms in Dali,Yunnan province.The complete nucleotide sequence of DNA-A was determined,it contained 2 739 nucleotides.Comparisons showed that DNA-A of YN72 had the highest sequence identity(93.4%) with that of Tomato yellow leaf curl China virus(TYLCCNV) isolate Y43,and 92.9% with that of TYLCCNV tobacco isolate Y5,while less than 90% identities were found when compared with other begomoviruses isolated in Asian.It was concluded that isolate YN72 infecting Datura stramonium L.was one of TYLCCNV isolates.Satellite DNA molecule 'YN72β' was found to be associated with YN72 using the primers beta01 and beta02.YN72β consisted of 1 335 nucleotides,with a functional ORF(C1) in complementary-sense DNA.It had 99.8% and 99.3% sequence identities with Beanβ and Y297β associated with TYLCCNV,respectively,and lower than 80.6% sequence identities were found with other reported satellite DNA molecules.Relationship dendrograms showed that satellite DNAβ molecules were co-evolved with their help begomoviruses.

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