Activity of Antioxidant Enzymes in Response to Cadmium in Oxya Chinensis

LI Li-jun1,2,GUO Ya-ping3,LIU Ping2,WANG Yue1,MA En-bo1 *(1.Institute of Applied Biology,Shanxi University,Taiyuan 030006,China;2.Institute of Agricultural Environment and Resource,Shanxi Academy of Agricultural Science,Taiyuan 030006,China;3.College of Life Science,Shanxi University,Taiyuan 030006,China;)

Oxya chinenesis;cadmium;superoxide dismutase(SOD);catalase(CAT);glutathione peroxidase(GPx);hydrogen peroxide(H2O2)

This paper was to present the influence of Cd on antioxidant enzymes in Oxya chinensis.The activities of superoxide dismutase(SOD),catalase(CAT),glutathione peroxidase(GPx),and the level of hydrogen peroxide(H2O2) were determined in O.chinensis which were fed for 30 d on wheat seedlings exposed to different Cd concentration(0.25(control),24.57,36.67,43.50 mg·kg-1 and 50.70 mg·kg-1).The results indicated that these enzymes activities and H2O2 level were varied in the presence of Cd,which suggested that there was an overproduction of reactive oxygen species(ROS) in O.chinensis following Cd treatment.In males,SOD activity declined by 19.86%,38.65%,28.98% and 71.49% respecting to control value,respectively,along with a decrease in CAT activity by 6.90%,68.70%,76.49% and 71.04% at 24.57,36.67,43.50 mg·kg-1 and 50.70 mg·kg-1 Cd in wheat seedlings,respectively.GPx activity increased by 56.73%,102.95%,70.17% at 24.57,36.67,43.50 mg·kg-1 Cd,respectively,decreased by 11.08% at 50.70 mg·kg-1 Cd,and H2O2 level increased by 13.61%,13.69% and 102.47% at 24.57,43.50 mg·kg-1 and 50.70 mg·kg-1 Cd,respectively,and declined by 27.05% at 36.67 mg·kg-1 Cd.In females,SOD activity increased by 64.29% and 23.29% at 24.57 mg·kg-1 and 50.70 mg·kg-1 Cd,decreased by 31.78% and 18.54% at 36.67 mg·kg-1 and 43.50 mg·kg-1 Cd.CAT activity declined by 8.73%,45.31%,9.98% and 5.17% at 24.57,36.67,43.50 mg·kg-1 and 50.70 mg·kg-1 Cd,respectively.GPx activity increased by 0.15% and 0.88% at 24.57 mg·kg-1 and 43.50 mg·kg-1 Cd,decreased by 20.50% and 44.40% at 36.67 mg·kg-1 and 50.70 mg·kg-1 Cd.H2O2 level elevated by 42.68%,and 26.01 % at 24.57 mg·kg-1 and 43.50 mg·kg-1,reduced by 15.10% and 13.97% at 36.67 mg·kg-1 and 50.70 mg·kg-1 Cd.The relationship between Cd toxicity and antioxidative system was complex,and antioxidant enzymes,such as SOD,CAT and GPx were involved in the mechanism to protect O.chinensis from ROS induced by Cd.Meanwhile,SOD,CAT and GPx played significant roles in cell defense against oxidative damage.