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Position: Home > Articles > Construction of Positive Plasmid for HA Detection Fragment of H9N2 Subtype of Avian Influenza Virus Acta Agriculturae Jiangxi 2019 (2) 86-90

H9N2亚型禽流感病毒HA检测片段阳性质粒的构建

作  者:
杨婧;刘宇卓;刘青涛;赵冬敏;章丽娇;黄欣梅;韩凯凯;李银
单  位:
江苏省农业科学院农业部兽用生物(兽医研究所)制品工程技术重点实验室
关键词:
禽流感病毒;H9N2;HA检测片段;阳性质粒;构建
摘  要:
本试验按照农业部NYT 772─2013标准构建了长度为487 bp的H9N2亚型禽流感病毒HA检测片段阳性质粒,并对该阳性质粒的特异性、敏感性和重复性进行了鉴定,获得了可用于检测H9N2亚型禽流感病毒HA片段的阳性质粒。该质粒在-20℃下即可保存,使用方便,不会污染环境,不存在散播病毒的危险。
译  名:
Construction of Positive Plasmid for HA Detection Fragment of H9N2 Subtype of Avian Influenza Virus
作  者:
YANG Jing;LIU Yu-zhuo;LIU Qing-tao;ZHAO Dong-min;ZHANG Li-jiao;HUANG Xin-mei;HAN Kai-kai;LI Yin;Institute of Veterinary Medicine,Jiangsu Academy of Agricultural Sciences/Key Laboratory of Veterinary Biological Product Engineering and Technology,Ministry of Agriculture;
单  位:
YANG Jing%LIU Yu-zhuo%LIU Qing-tao%ZHAO Dong-min%ZHANG Li-jiao%HUANG Xin-mei%HAN Kai-kai%LI Yin%Institute of Veterinary Medicine,Jiangsu Academy of Agricultural Sciences/Key Laboratory of Veterinary Biological Product Engineering and Technology,Ministry of Agriculture
关键词:
Avian influenza virus;;H9N2;;Detection fragment of HA;;Positive plasmid;;Construction
摘  要:
According to the NYT 772─2013 standard issued by Ministry of Agriculture,we constructed a 487-bp-long positive plasmid for detecting the HA fragment of H9 N2 subtype of avian influenza virus,identified the specificity,sensitivity and repeatability of this positive plasmid,and successfully obtained the positive plasmid which can be used to detect the HA fragment of H9 N2-subtype avian influenza virus. This positive plasmid can be stored at-20 ℃ and is easy to be used,and it has not dangers of spreading virus and polluting the environment.

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